Reversible protein inactivation by optogenetic trapping in cells Highly Cited Paper

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Title
Reversible protein inactivation by optogenetic trapping in cells
Author(s)
Sangkyu Lee; Hyerim Park; Taeyoon Kyung; Na Yeon Kim; Sungsoo Kim; Jihoon Kim; Won Do Heo
Publication Date
2014-06
Journal
NATURE METHODS, v.11, no.6, pp.633 - 636
Publisher
NATURE PUBLISHING GROUP
Abstract
We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP. © 2014 Nature America, Inc.
URI
https://pr.ibs.re.kr/handle/8788114/1507
ISSN
1548-7091
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
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