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IBS Publications RepositoryCenter for Cognition and Sociality(인지 및 사회성 연구단)1. Journal Papers (저널논문)

Reversible protein inactivation by optogenetic trapping in cells

DC Field	Value	Language
dc.contributor.author	Sangkyu Lee	-
dc.contributor.author	Hyerim Park	-
dc.contributor.author	Taeyoon Kyung	-
dc.contributor.author	Na Yeon Kim	-
dc.contributor.author	Sungsoo Kim	-
dc.contributor.author	Jihoon Kim	-
dc.contributor.author	Won Do Heo	-
dc.date.available	2015-04-21T09:15:20Z	-
dc.date.created	2014-08-11	-
dc.date.issued	2014-06	-
dc.identifier.issn	1548-7091	-
dc.identifier.uri	https://pr.ibs.re.kr/handle/8788114/1507	-
dc.description.abstract	We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP. © 2014 Nature America, Inc.	-
dc.description.uri	1	-
dc.language	영어	-
dc.publisher	NATURE PUBLISHING GROUP	-
dc.subject	calcium calmodulin dependent protein kinase II	-
dc.subject	cryptochrome 2	-
dc.subject	epitope	-
dc.subject	nanobody	-
dc.subject	phosphatidylinositol 3 kinase	-
dc.subject	Arabidopsis thaliana	-
dc.subject	article	-
dc.subject	cell function	-
dc.subject	cell polarity	-
dc.subject	cell structure	-
dc.subject	cell viability	-
dc.subject	cytoskeleton	-
dc.subject	endosome	-
dc.subject	HeLa cell line	-
dc.subject	human	-
dc.subject	human cell	-
dc.subject	light activated reversible inhibition by assembled trap	-
dc.subject	mammal cell	-
dc.subject	microtubule	-
dc.subject	nonhuman	-
dc.subject	optogenetics	-
dc.subject	priority journal	-
dc.subject	protein expression	-
dc.subject	protein function	-
dc.subject	protein processing	-
dc.subject	reversible protein inactivation	-
dc.subject	signal transduction	-
dc.title	Reversible protein inactivation by optogenetic trapping in cells	-
dc.type	Article	-
dc.type.rims	ART	-
dc.identifier.wosid	000336899300020	-
dc.identifier.scopusid	2-s2.0-84901843462	-
dc.identifier.rimsid	13	ko
dc.date.tcdate	2018-10-01	-
dc.contributor.affiliatedAuthor	Sangkyu Lee	-
dc.contributor.affiliatedAuthor	Won Do Heo	-
dc.identifier.doi	10.1038/nmeth.2940	-
dc.identifier.bibliographicCitation	NATURE METHODS, v.11, no.6, pp.633 - 636	-
dc.citation.title	NATURE METHODS	-
dc.citation.volume	11	-
dc.citation.number	6	-
dc.citation.startPage	633	-
dc.citation.endPage	636	-
dc.date.scptcdate	2018-10-01	-
dc.description.wostc	72	-
dc.description.scptc	75	-
dc.description.journalClass	1	-
dc.description.journalRegisteredClass	scie	-
dc.description.journalRegisteredClass	scopus	-