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Reversible protein inactivation by optogenetic trapping in cells

DC Field Value Language
dc.contributor.authorSangkyu Lee-
dc.contributor.authorHyerim Park-
dc.contributor.authorTaeyoon Kyung-
dc.contributor.authorNa Yeon Kim-
dc.contributor.authorSungsoo Kim-
dc.contributor.authorJihoon Kim-
dc.contributor.authorWon Do Heo-
dc.date.available2015-04-21T09:15:20Z-
dc.date.created2014-08-11-
dc.date.issued2014-06-
dc.identifier.issn1548-7091-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/1507-
dc.description.abstractWe present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP. © 2014 Nature America, Inc.-
dc.description.uri1-
dc.language영어-
dc.publisherNATURE PUBLISHING GROUP-
dc.subjectcalcium calmodulin dependent protein kinase II-
dc.subjectcryptochrome 2-
dc.subjectepitope-
dc.subjectnanobody-
dc.subjectphosphatidylinositol 3 kinase-
dc.subjectArabidopsis thaliana-
dc.subjectarticle-
dc.subjectcell function-
dc.subjectcell polarity-
dc.subjectcell structure-
dc.subjectcell viability-
dc.subjectcytoskeleton-
dc.subjectendosome-
dc.subjectHeLa cell line-
dc.subjecthuman-
dc.subjecthuman cell-
dc.subjectlight activated reversible inhibition by assembled trap-
dc.subjectmammal cell-
dc.subjectmicrotubule-
dc.subjectnonhuman-
dc.subjectoptogenetics-
dc.subjectpriority journal-
dc.subjectprotein expression-
dc.subjectprotein function-
dc.subjectprotein processing-
dc.subjectreversible protein inactivation-
dc.subjectsignal transduction-
dc.titleReversible protein inactivation by optogenetic trapping in cells-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000336899300020-
dc.identifier.scopusid2-s2.0-84901843462-
dc.identifier.rimsid13ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorSangkyu Lee-
dc.contributor.affiliatedAuthorWon Do Heo-
dc.identifier.doi10.1038/nmeth.2940-
dc.identifier.bibliographicCitationNATURE METHODS, v.11, no.6, pp.633 - 636-
dc.citation.titleNATURE METHODS-
dc.citation.volume11-
dc.citation.number6-
dc.citation.startPage633-
dc.citation.endPage636-
dc.date.scptcdate2018-10-01-
dc.description.wostc72-
dc.description.scptc75-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > 1. Journal Papers (저널논문)
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