Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling

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Title
Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling
Author(s)
Ki-Young Chang; Doyeon Woo; Hyunjin Jung; Sangkyu Lee; Sungsoo Kim; Joungha Won; Taeyoon Kyung; Hyerim Park; Nury Kim; Hee Won Yang; Jae-Yong Park; Eun Mi Hwang; Daesoo Kim; Won Do Heo
Publication Date
2014-06
Journal
NATURE COMMUNICATIONS, v.5, no., pp.4057 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Receptor tyrosine kinases (RTKs) are a family of cell-surface receptors that have a key role in regulating critical cellular processes. Here, to understand and precisely control RTK signalling, we report the development of a genetically encoded, photoactivatable Trk (tropomyosin-related kinase) family of RTKs using a light-responsive module based on Arabidopsis thaliana cryptochrome 2. Blue-light stimulation (488-nm) of mammalian cells harbouring these receptors robustly upregulates canonical Trk signalling. A single light stimulus triggers transient signalling activation, which is reversibly tuned by repetitive delivery of blue-light pulses. In addition, the light-provoked process is induced in a spatially restricted and cell-specific manner. A prolonged patterned illumination causes sustained activation of extracellular signal-regulated kinase and promotes neurite outgrowth in a neuronal cell line, and induces filopodia formation in rat hippocampal neurons. These light-controllable receptors are expected to create experimental opportunities to spatiotemporally manipulate many biological processes both in vitro and in vivo. © 2014 Macmillan Publishers Limited.
URI
https://pr.ibs.re.kr/handle/8788114/1506
ISSN
2041-1723
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
2014_Nature_Communications.pdfDownload

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