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Upgrading interferometric scattering microscopy with ensemble statistical analysis

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Title
Upgrading interferometric scattering microscopy with ensemble statistical analysis
Author(s)
Minsu Lee; Seok-Cheol Hong; Minhaeng Cho
Publication Date
2024-02
Journal
Bulletin of the Korean Chemical Society, v.45, no.1, pp.32 - 44
Publisher
대한화학회
Abstract
Interferometric scattering (iSCAT) microscopy, label-free high-speed (up to ~1000 frames per second) imaging and tracking technique, has proven to be a versatile tool by measuring the mass and 3D position of nanoparticles and biomolecules as well as visualizing real-time dynamics of nanoscale events in complex cellular environments. However, the quantification of iSCAT signals has not been straightforwardly defined in practical terms. We delve into several issues associated with signal processing in iSCAT: error-prone post-processing routine and lack of statistical reliability in the convention of iSCAT contrast. After providing a brief account of concepts and principles of correlation spectroscopy, we here discuss an alternative ensemble (higher number density of scatterers) statistical analysis that can be used to extract the dynamic information of scattering particles from fluctuating iSCAT signals. Finally, our perspective on the correlation approach toward time-correlated iSCAT technique will be presented.
URI
https://pr.ibs.re.kr/handle/8788114/14780
DOI
10.1002/bkcs.12800
ISSN
0253-2964
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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