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분자분광학및동력학연구단
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Upgrading interferometric scattering microscopy with ensemble statistical analysis

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dc.contributor.authorMinsu Lee-
dc.contributor.authorSeok-Cheol Hong-
dc.contributor.authorMinhaeng Cho-
dc.date.accessioned2024-02-06T22:00:45Z-
dc.date.available2024-02-06T22:00:45Z-
dc.date.created2023-12-05-
dc.date.issued2024-02-
dc.identifier.issn0253-2964-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/14780-
dc.description.abstractInterferometric scattering (iSCAT) microscopy, label-free high-speed (up to ~1000 frames per second) imaging and tracking technique, has proven to be a versatile tool by measuring the mass and 3D position of nanoparticles and biomolecules as well as visualizing real-time dynamics of nanoscale events in complex cellular environments. However, the quantification of iSCAT signals has not been straightforwardly defined in practical terms. We delve into several issues associated with signal processing in iSCAT: error-prone post-processing routine and lack of statistical reliability in the convention of iSCAT contrast. After providing a brief account of concepts and principles of correlation spectroscopy, we here discuss an alternative ensemble (higher number density of scatterers) statistical analysis that can be used to extract the dynamic information of scattering particles from fluctuating iSCAT signals. Finally, our perspective on the correlation approach toward time-correlated iSCAT technique will be presented.-
dc.language영어-
dc.publisher대한화학회-
dc.titleUpgrading interferometric scattering microscopy with ensemble statistical analysis-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid001116463900001-
dc.identifier.scopusid2-s2.0-85177553342-
dc.identifier.rimsid82128-
dc.contributor.affiliatedAuthorMinsu Lee-
dc.contributor.affiliatedAuthorSeok-Cheol Hong-
dc.contributor.affiliatedAuthorMinhaeng Cho-
dc.identifier.doi10.1002/bkcs.12800-
dc.identifier.bibliographicCitationBulletin of the Korean Chemical Society, v.45, no.1, pp.32 - 44-
dc.relation.isPartOfBulletin of the Korean Chemical Society-
dc.citation.titleBulletin of the Korean Chemical Society-
dc.citation.volume45-
dc.citation.number1-
dc.citation.startPage32-
dc.citation.endPage44-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.relation.journalWebOfScienceCategoryChemistry, Multidisciplinary-
dc.subject.keywordPlusDIFFUSION-
dc.subject.keywordPlusPOSITION-
dc.subject.keywordPlusPHASE-
dc.subject.keywordPlusFLOW-
dc.subject.keywordPlusFLUORESCENCE CORRELATION SPECTROSCOPY-
dc.subject.keywordPlusLABEL-FREE-
dc.subject.keywordPlusLIGHT-SCATTERING-
dc.subject.keywordPlusFLUCTUATION SPECTROSCOPY-
dc.subject.keywordPlusPARTICLE TRACKING-
dc.subject.keywordPlusORIENTATION-
dc.subject.keywordAuthordynamic light scattering-
dc.subject.keywordAuthorfluorescence correlation spectroscopy-
dc.subject.keywordAuthorinterferometric scattering microscopy-
dc.subject.keywordAuthorlive cell-
dc.subject.keywordAuthorRayleigh scattering-
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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