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나노물질 및 화학반응 연구단
나노물질 및 화학반응 연구단
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Simple super-resolution live-cell imaging based on diffusion-assisted Forster resonance energy transfer

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Title
Simple super-resolution live-cell imaging based on diffusion-assisted Forster resonance energy transfer
Author(s)
Cho, Sangyeon; Jang, Jaeduck; Song, Chaeyeon; Lee, Heeyoung; Prabhakar Ganesan; Yoon, Tae-Young; Kim, Mahn Won; Choi, Myung Chul; Hyot Cherl Ihee; Heo, Won Do; Park, YongKeun
Publication Date
2013-02
Journal
SCIENTIFIC REPORTS, v.3, no., pp.1208 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Despite the recent development of several super-resolution fluorescence microscopic techniques, there are still few techniques that can be readily employed in conventional imaging systems.Wepresent a very simple, rapid, general and cost-efficient super-resolution imaging method, which can be directly employed in a simple fluorescent imaging system with general fluorophores. Based on diffusion-assisted Fo¨rster resonance energy transfer (FRET), fluorescent donor molecules that label specific target structures can be stochastically quenched by diffusing acceptor molecules, thereby temporally separating otherwise spatially overlapped fluorescence signals and allowing super-resolution imaging. The proposed method provides two- to three-fold-enhancement in spatial resolution, a significant optical sectioning property, and favorable temporal resolution in live-cell imaging. We demonstrate super-resolution live-cell dynamic imaging using general fluorophores in a standard epi-fluorescence microscope with light-emitting diode (LED) illumination. Due to the simplicity of this approach, we expect that the proposed method will prove an attractive option for super-resolution imaging.
URI
http://pr.ibs.re.kr/handle/8788114/1383
DOI
10.1038/srep01208
ISSN
2045-2322
Appears in Collections:
Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > Journal Papers (저널논문)
Files in This Item:
20_IBS CA1201-1_Scientific Reports_3_01_1208_Simple super-resolution live-cell imaging based on diffusion-assisted Forster resonance energy.pdfDownload

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