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나노물질및화학반응연구단
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Simple super-resolution live-cell imaging based on diffusion-assisted Forster resonance energy transfer

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dc.contributor.authorCho, Sangyeon-
dc.contributor.authorJang, Jaeduck-
dc.contributor.authorSong, Chaeyeon-
dc.contributor.authorLee, Heeyoung-
dc.contributor.authorPrabhakar Ganesan-
dc.contributor.authorYoon, Tae-Young-
dc.contributor.authorKim, Mahn Won-
dc.contributor.authorChoi, Myung Chul-
dc.contributor.authorHyot Cherl Ihee-
dc.contributor.authorHeo, Won Do-
dc.contributor.authorPark, YongKeun-
dc.date.available2015-04-20T07:15:11Z-
dc.date.created2014-08-11ko
dc.date.issued2013-02-
dc.identifier.issn2045-2322-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/1383-
dc.description.abstractDespite the recent development of several super-resolution fluorescence microscopic techniques, there are still few techniques that can be readily employed in conventional imaging systems.Wepresent a very simple, rapid, general and cost-efficient super-resolution imaging method, which can be directly employed in a simple fluorescent imaging system with general fluorophores. Based on diffusion-assisted Fo¨rster resonance energy transfer (FRET), fluorescent donor molecules that label specific target structures can be stochastically quenched by diffusing acceptor molecules, thereby temporally separating otherwise spatially overlapped fluorescence signals and allowing super-resolution imaging. The proposed method provides two- to three-fold-enhancement in spatial resolution, a significant optical sectioning property, and favorable temporal resolution in live-cell imaging. We demonstrate super-resolution live-cell dynamic imaging using general fluorophores in a standard epi-fluorescence microscope with light-emitting diode (LED) illumination. Due to the simplicity of this approach, we expect that the proposed method will prove an attractive option for super-resolution imaging.-
dc.description.uri1-
dc.language영어-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleSimple super-resolution live-cell imaging based on diffusion-assisted Forster resonance energy transfer-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000314401900003-
dc.identifier.scopusid2-s2.0-84873666238-
dc.identifier.rimsid85ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorPrabhakar Ganesan-
dc.contributor.affiliatedAuthorHyot Cherl Ihee-
dc.identifier.doi10.1038/srep01208-
dc.identifier.bibliographicCitationSCIENTIFIC REPORTS, v.3, pp.1208-
dc.citation.titleSCIENTIFIC REPORTS-
dc.citation.volume3-
dc.citation.startPage1208-
dc.date.scptcdate2018-10-01-
dc.description.wostc24-
dc.description.scptc30-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
Appears in Collections:
Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > 1. Journal Papers (저널논문)
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20_IBS CA1201-1_Scientific Reports_3_01_1208_Simple super-resolution live-cell imaging based on diffusion-assisted Forster resonance energy.pdfDownload

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