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나노 구조 물리 연구단
나노구조물리 연구단
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Direct high-resolution label-free imaging of cellular nanostructure dynamics in living cells

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Title
Direct high-resolution label-free imaging of cellular nanostructure dynamics in living cells
Author(s)
Chaejeong Heo; Sohee Lee; Si Young Lee; Mun Seok Jeong; Lee, Young Hee; Minah Suh
Publication Date
2013-06
Journal
JOURNAL OF BIOMEDICAL OPTICS, v.18, no.6, pp.66016 -
Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
Abstract
We report the application of an optical microscope equipped with a high-resolution dark-field condenser for detecting dynamic responses of cellular nanostructures in real time. Our system provides an easy-to-use technique to visualize biological specimens without any staining. This system can visualize the dynamic behavior of nanospheres and nanofibers, such as F-actin, at the leading edges of adjacent neuronal cells. We confirmed that the nanofibers imaged with this high-resolution optical microscopic technique are F-actin by using fluorescence microscopy after immunostaining the F-actin of fixed cells. Furthermore, cellular dynamics are enhanced by applying noncontact electric field stimulation through a transparent graphene electric field stimulator. High-resolution label-free optical microscopy enables the visualization of nanofiber dynamics initiated by filopodial nanofiber contacts. In conclusion, our optical microscopy system allows the visualization of nanoscale cellular dynamics under various external stimuli in real time without specific staining. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
URI
http://pr.ibs.re.kr/handle/8788114/1318
DOI
10.1117/1.JBO.18.6.066016
ISSN
1083-3668
Appears in Collections:
Center for Integrated Nanostructure Physics(나노구조물리 연구단) > Journal Papers (저널논문)
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