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Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues

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Title
Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues
Author(s)
Wonjun Choi; Munkyu Kang; Jin Hee Hong; Ori Katz; Byunghak Lee; Guang Hoon Kim; Youngwoon Choi; Wonshik Choi
Publication Date
2022-12
Journal
NATURE COMMUNICATIONS, v.13, no.1
Publisher
NATURE PORTFOLIO
Abstract
Lensless fibre endoscopes are minimally invasive, but are often rigid and require calibration and fluorescence labelling. Here, the authors present a flexible endoscope based on a bare fibre bundle and a lensless Fourier holographic imaging configuration to detect weak reflections from unstained biological tissues. Ultrathin lensless fibre endoscopes offer minimally invasive investigation, but they mostly operate as a rigid type due to the need for prior calibration of a fibre probe. Furthermore, most implementations work in fluorescence mode rather than label-free imaging mode, making them unsuitable for general medical diagnosis. Herein, we report a fully flexible ultrathin fibre endoscope taking 3D holographic images of unstained tissues with 0.85-mu m spatial resolution. Using a bare fibre bundle as thin as 200-mu m diameter, we design a lensless Fourier holographic imaging configuration to selectively detect weak reflections from biological tissues, a critical step for label-free endoscopic reflectance imaging. A unique algorithm is developed for calibration-free holographic image reconstruction, allowing us to image through a narrow and curved passage regardless of fibre bending. We demonstrate endoscopic reflectance imaging of unstained rat intestine tissues that are completely invisible to conventional endoscopes. The proposed endoscope will expedite a more accurate and earlier diagnosis than before with minimal complications.
URI
https://pr.ibs.re.kr/handle/8788114/12272
DOI
10.1038/s41467-022-32114-5
ISSN
2041-1723
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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