Nitric oxide integrated polyethylenimine-based tri-block copolymer for efficient antibacterial activity

Abstract

The work demonstrated a successful synthesis of nitric oxide (NO)-releasing material and its antibacterial effect on Gram-negative Escherichia coli (E. coli), Gram-positive Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA). The polymeric support composed of thermosensitive Pluronic F68 having good biocompatibility and branched polyethylenimine (BPEI) housed N-diazeniumdiolates (NONOates) which could store and release NO under appropriate physiological condition. The developed F68eBPEIeNONOates releases a suf!cient amount of NO under physiological condition to elicit effective killing of E. coli, S. aureus and MRSA. The antibacterial ability of the released NO was compared to un- treated control or unmodi!ed F68 polymer by using confocal microscopy; F68eBPEIeNONOates demonstrated excellent antibacterial activity with in vitro low cytotoxicity. TEM investigation also revealed the destruction of bacteria membrane caused by NO. The effectiveness of F68eBPEIeNONOates against resistant strains such as MRSA provides a very simple but highly ef!cient strategy to combat drug-resistant bacterial infections.