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나노입자 연구단
나노입자 연구단
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A Rapid Antimicrobial Susceptibility Test Based on Single-Cell Morphological Analysis

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Title
A Rapid Antimicrobial Susceptibility Test Based on Single-Cell Morphological Analysis
Author(s)
Jungil Choi; Jungheon Yoo; Mincheol Lee; Eun-Geun Kim; Ji Soo Lee; Seungok Lee; Seik Joo; Sang Hoon Song; Eui-Chong Kim; Jung Chan Lee; Hee Chan Kim; Yong-Gyun Jung; Sunghoon Kwon
Publication Date
2014-12
Journal
SCIENCE TRANSLATIONAL MEDICINE, v.6, no.267, pp. -
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
Abstract
A rapid antibiotic susceptibility test (AST) is desperately needed in clinical settings for fast and appropriate antibiotic administration. Traditional ASTs, which rely on cell culture, are not suitable for urgent cases of bacterial infection and antibiotic resistance owing to their relatively long test times. We describe a novel AST called single-cell morphological analysis (SCMA) that can determine antimicrobial susceptibility by automatically analyzing and categorizing morphological changes in single bacterial cells under various antimicrobial conditions. The SCMAwas tested with four Clinical and Laboratory Standards Institute standard bacterial strains and 189 clinical samples, including extended-spectrum b-lactamase–positive Escherichia coli and Klebsiella pneumoniae, imipenem-resistant Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and vancomycin-resistant Enterococci from hospitals. The results were compared with the gold standard broth microdilution test. The SCMA results were obtained in less than 4 hours, with 91.5% categorical agreement and 6.51% minor, 2.56% major, and 1.49% very major discrepancies. Thus, SCMA provides rapid and accurate antimicrobial susceptibility data that satisfy the recommended performance of the U.S. Food and Drug Administration.
URI
https://pr.ibs.re.kr/handle/8788114/1185
ISSN
1946-6234
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > Journal Papers (저널논문)
Files in This Item:
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