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인지 및 사회성 연구단
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Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission, and behavior

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Title
Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission, and behavior
Author(s)
Joungha Won; Yuriy Pankratov; Minwoo Wendy Jang; Sunpil Kim; Yeon Ha Ju; Sangkyu Lee; Seung Eun Lee; Arie Kim; Soowon Park; C. Justin Lee; Won Do Heo
Publication Date
2022-02
Journal
Neuron, v.110, no.3, pp.423 - 435
Publisher
Cell Press
Abstract
Spatiotemporal control of brain activity by optogenetics has emerged as an essential tool to study brain function. For silencing brain activity, optogenetic probes, such as halorhodopsin and archaerhodopsin, inhibit transmitter release indirectly by hyperpolarizing membrane potentials. However, these probes cause an undesirable ionic imbalance and rebound spikes. Moreover, they are not applicable to use in non-excitable glial cells. Here we engineered Opto-vTrap, a light-inducible and reversible inhibition system to temporarily trap the transmitter-containing vesicles from exocytotic release. Light activation of Opto-vTrap caused full vesicle clusterization and complete inhibition of exocytosis within 1min,which recovered within 30min after light off.We found a significant reduction in synaptic and gliotransmission upon activation of Opto-vTrap in acute brain slices. Opto-vTrap significantly inhibited hippocampus-dependent memory retrieval with full recovery within an hour. We propose Opto-vTrap as a next-generation optogenetic silencer to control brain activity and behavior with minimal confounding effects.
URI
https://pr.ibs.re.kr/handle/8788114/11732
DOI
10.1016/j.neuron.2021.11.003
ISSN
0896-6273
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > 1. Journal Papers (저널논문)
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