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Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids

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Title
Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids
Author(s)
Suran Kim; Sungjin Min; Yi Sun Choi; Sung-Hyun Jo; Jae Hun Jung; Kyusun Han; Jin Kim; Soohwan An; Yong Woo Ji; Yun-Gon Kim; Seung-Woo Cho
Publication Date
2022-03
Journal
Nature Communications, v.13, no.1
Publisher
Nature Research
Abstract
© 2022, The Author(s).Matrigel, a mouse tumor extracellular matrix protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to-batch variation, high cost, and safety issues. Here, we demonstrate that gastrointestinal tissue-derived extracellular matrix hydrogels are suitable substitutes for Matrigel in gastrointestinal organoid culture. We found that the development and function of gastric or intestinal organoids grown in tissue extracellular matrix hydrogels are comparable or often superior to those in Matrigel. In addition, gastrointestinal extracellular matrix hydrogels enabled long-term subculture and transplantation of organoids by providing gastrointestinal tissue-mimetic microenvironments. Tissue-specific and age-related extracellular matrix profiles that affect organoid development were also elucidated through proteomic analysis. Together, our results suggest that extracellular matrix hydrogels derived from decellularized gastrointestinal tissues are effective alternatives to the current gold standard, Matrigel, and produce organoids suitable for gastrointestinal disease modeling, drug development, and tissue regeneration.
URI
https://pr.ibs.re.kr/handle/8788114/11455
DOI
10.1038/s41467-022-29279-4
ISSN
2041-1723
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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