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Fluorescent probe strategy for live cell distinction

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Title
Fluorescent probe strategy for live cell distinction
Author(s)
Liu, Xiao; Young-Tae Chang
Publication Date
2022-03-07
Journal
CHEMICAL SOCIETY REVIEWS, v.51, no.5, pp.1573 - 1591
Publisher
ROYAL SOC CHEMISTRY
Abstract
Live cell discrimination is the first and essential step to understand complex biosystems. Conventional cell discrimination involving various antibodies relies on selective surface biomarkers. Compared to antibodies, the fluorescent probe strategy allows the utilisation of intracellular biomarkers, providing broader options with unique chemical principles to achieve the live cell distinction. In general, fluorescent probes can be retained in cells by interacting with biomolecules, accumulating via transporters, and participating in metabolism. Based on the target difference, fluorescent probe strategy can be divided into several categories: protein-oriented live cell distinction (POLD), carbohydrate-oriented live cell distinction (COLD), DNA-oriented live cell distinction (DOLD), gating-oriented live cell distinction (GOLD), metabolism-oriented live cell distinction (MOLD) and lipid-oriented live cell distinction (LOLD). In this review, we will outline the concepts and mechanisms of different strategies, introduce their applications in cell-type discrimination, and discuss their advantages and challenges in this area. We expect this tutorial will provide a new perspective on the mechanisms of fluorescent probe strategy and facilitate the development of cell-type-specific probes.
URI
https://pr.ibs.re.kr/handle/8788114/11255
DOI
10.1039/d1cs00388g
ISSN
0306-0012
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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