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Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

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Title
Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain
Author(s)
Myungjae Song; Jung Min Lim; Seonwoo Min; Jeong-Seok Oh; Dong Young Kim; Jae-Sung Woo; Hiroshi Nishimasu; Sung-Rae Cho; Sungroh Yoon; Hyongbum Henry Kim
Publication Date
2021-09
Journal
Nature Communications, v.12, no.1, pp.1 - 8
Publisher
Nature Publishing Group
Abstract
© 2021, The Author(s).Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.
URI
https://pr.ibs.re.kr/handle/8788114/10803
DOI
10.1038/s41467-021-25928-2
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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