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나노의학 연구단
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Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

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dc.contributor.authorMyungjae Song-
dc.contributor.authorJung Min Lim-
dc.contributor.authorSeonwoo Min-
dc.contributor.authorJeong-Seok Oh-
dc.contributor.authorDong Young Kim-
dc.contributor.authorJae-Sung Woo-
dc.contributor.authorHiroshi Nishimasu-
dc.contributor.authorSung-Rae Cho-
dc.contributor.authorSungroh Yoon-
dc.contributor.authorHyongbum Henry Kim-
dc.date.accessioned2021-12-08T06:50:07Z-
dc.date.available2021-12-08T06:50:07Z-
dc.date.created2021-10-05-
dc.date.issued2021-09-23-
dc.identifier.issn2041-1723-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/10803-
dc.description.abstract© 2021, The Author(s).Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.-
dc.language영어-
dc.publisherNature Publishing Group-
dc.titleGeneration of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000698773500004-
dc.identifier.scopusid2-s2.0-85115650595-
dc.identifier.rimsid76453-
dc.contributor.affiliatedAuthorHyongbum Henry Kim-
dc.identifier.doi10.1038/s41467-021-25928-2-
dc.identifier.bibliographicCitationNature Communications, v.12, no.1, pp.1 - 8-
dc.relation.isPartOfNature Communications-
dc.citation.titleNature Communications-
dc.citation.volume12-
dc.citation.number1-
dc.citation.startPage1-
dc.citation.endPage8-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.relation.journalWebOfScienceCategoryMultidisciplinary Sciences-
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Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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