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Hyperspectral fluorescence imaging for cellular iron mapping in the in vitro model of Parkinson's disease

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Title
Hyperspectral fluorescence imaging for cellular iron mapping in the in vitro model of Parkinson's disease
Author(s)
Oh E.S.; Chaejeong Heo; Kim J.S.; Minah Suh; Young Hee Lee; Kim J.-M.
Publication Date
2014-05
Journal
JOURNAL OF BIOMEDICAL OPTICS, v.19, no.5, pp.51207 -
Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
Abstract
Parkinson's disease (PD) is characterized by progressive dopaminergic cell loss in the substantia nigra (SN) and elevated iron levels demonstrated by autopsy. Direct visualization of iron with live imaging techniques has not yet been successful. The aim of this study is to visualize and quantify the distribution of cellular iron using an intrinsic iron hyperspectral fluorescence signal. The 1-methyl-4-phenylpyridinium (MPP+)-induced cellular model of PD was established in SHSY5Y cells exposed to iron with ferric ammonium citrate (FAC, 100 ìM). The hyperspectral fluorescence signal of iron was examined using a high-resolution dark-field optical microscope system with signal absorption for the visible/near infrared spectral range. The 6-h group showed heavy cellular iron deposition compared with the 1-h group. The cellular iron was dispersed in a small particulate form, whereas the extracellular iron was aggregated. In addition, iron particles were found to be concentrated on the cell membrane/edge of shrunken cells. The iron accumulation readily occurred in MPP+-induced cells, which is consistent with previous studies demonstrating elevated iron levels in the SN. This direct iron imaging could be applied to analyze the physiological role of iron, and its application might be expanded to various neurological disorders involving metals, such as copper, manganese, or zinc. © The Authors.
URI
http://pr.ibs.re.kr/handle/8788114/1023
DOI
10.1117/1.JBO.19.5.051207
ISSN
1083-3668
Appears in Collections:
Center for Integrated Nanostructure Physics(나노구조물리 연구단) > Journal Papers (저널논문)
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > Journal Papers (저널논문)
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