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복잡계 자기조립 연구단
복잡계 자기조립 연구단
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i-Motif-Driven Au Nanomachines in Programmed siRNA Delivery for Gene-Silencing and Photothermal Ablation

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Title
i-Motif-Driven Au Nanomachines in Programmed siRNA Delivery for Gene-Silencing and Photothermal Ablation
Author(s)
Sejin Son; Nam J.; Jinhwan Kim; Kim S.; Won Jong Kim
Publication Date
2014-06
Journal
ACS NANO, v.8, no.6, pp.5574 - 5584
Publisher
AMER CHEMICAL SOC
Abstract
The present work illustrates unique design, construction and operation of an i-motif-based DNA nanomachine templated on gold nanoparticles (AuNPs), which utilizes pH-responsive dynamic motion of i-motif DNA strands and aggregational behavior of AuNPs to elicit programmed delivery of therapeutic siRNA. The pH-sensitive nucleic acids immobilized on the AuNPs consisted of three functional segments, i.e., an i-motif DNA, an overhanging linker DNA and a therapeutic siRNA. At neutral pH, the i-motif DNA is hybridized with the overhanging linker DNA segment of the therapeutic siRNA. However, in endosomal acidic pH, the i-motif DNA forms interstrand tetraplex, which could induce cluster formation of AuNPs resulting in endosomal escape of AuNP clusters, and produce a high gene silencing efficiency by releasing siRNA in the cytosol. Furthermore, the cluster formation of AuNPs accelerated photothermal ablation of cells when irradiated with laser. Precise and synchronized biomechanical motion in subcellular microenvironment is realized through judicious integration of pH-responsive behavior of the i-motif DNA and AuNPs, and meticulous designing of DNA. © 2014 American Chemical Society.
URI
https://pr.ibs.re.kr/handle/8788114/1007
ISSN
1936-0851
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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