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Ki, Ho Sung
나노물질 및 화학반응 연구단
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Uncovering the Conformational Distribution of a Small Protein with Nanoparticle-Aided Cryo-Electron Microscopy Sampling

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Title
Uncovering the Conformational Distribution of a Small Protein with Nanoparticle-Aided Cryo-Electron Microscopy Sampling
Author(s)
Hosung Ki; Junbeom Jo; Youngmin Kim; Tae Wu Kim; Changin Kim; Kim, Yeeun; Kim, Chang Woo; Srinivasan Muniyappan; Sang Jin Lee; Yonggwan Kim; Ho Min Kim; Yang, Yongsoo; Rhee, Young Min; Hyotcherl Ihee
Publication Date
2021-07
Journal
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, v.12, no.28, pp.6565 - 6573
Publisher
AMER CHEMICAL SOC
Abstract
Here, we introduce the nanoparticle-aided cryo-electron microscopy sampling (NACS) method to access the conformational distribution of a protein molecule. Two nanogold particles are labeled at two target sites, and the interparticle distance is measured as a structural parameter via cryo-electron microscopy (cryo-EM). The key aspect of NACS is that the projected distance information instead of the global conformational information is extracted from each protein molecule. This is possible because the contrast provided by the nanogold particles is strong enough to provide the projected distance, while the protein itself is invisible due to its low contrast. We successfully demonstrate that various protein conformations, even for small or disordered proteins, which generally cannot be accessed via cryo-EM, can be captured. The demonstrated method with the potential to directly observe the conformational distribution of such systems may open up new possibilities in studying their dynamics at a single-molecule level.
URI
https://pr.ibs.re.kr/handle/8788114/10052
DOI
10.1021/acs.jpclett.1c01277
ISSN
1948-7185
Appears in Collections:
Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > 1. Journal Papers (저널논문)
Pioneer Research Center for Biomolecular and Cellular Structure(바이오분자 및 세포구조 연구단) > Protein Communication Group(단백질 커뮤니케이션 그룹) > 1. Journal Papers (저널논문)
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