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나노물질 및 화학반응 연구단
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Uncovering the Conformational Distribution of a Small Protein with Nanoparticle-Aided Cryo-Electron Microscopy Sampling

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dc.contributor.authorHosung Ki-
dc.contributor.authorJunbeom Jo-
dc.contributor.authorYoungmin Kim-
dc.contributor.authorTae Wu Kim-
dc.contributor.authorChangin Kim-
dc.contributor.authorKim, Yeeun-
dc.contributor.authorKim, Chang Woo-
dc.contributor.authorSrinivasan Muniyappan-
dc.contributor.authorSang Jin Lee-
dc.contributor.authorYonggwan Kim-
dc.contributor.authorHo Min Kim-
dc.contributor.authorYang, Yongsoo-
dc.contributor.authorRhee, Young Min-
dc.contributor.authorHyotcherl Ihee-
dc.date.accessioned2021-08-10T00:50:07Z-
dc.date.accessioned2021-08-10T00:50:07Z-
dc.date.available2021-08-10T00:50:07Z-
dc.date.available2021-08-10T00:50:07Z-
dc.date.created2021-08-09-
dc.date.issued2021-07-22-
dc.identifier.issn1948-7185-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/10052-
dc.description.abstractHere, we introduce the nanoparticle-aided cryo-electron microscopy sampling (NACS) method to access the conformational distribution of a protein molecule. Two nanogold particles are labeled at two target sites, and the interparticle distance is measured as a structural parameter via cryo-electron microscopy (cryo-EM). The key aspect of NACS is that the projected distance information instead of the global conformational information is extracted from each protein molecule. This is possible because the contrast provided by the nanogold particles is strong enough to provide the projected distance, while the protein itself is invisible due to its low contrast. We successfully demonstrate that various protein conformations, even for small or disordered proteins, which generally cannot be accessed via cryo-EM, can be captured. The demonstrated method with the potential to directly observe the conformational distribution of such systems may open up new possibilities in studying their dynamics at a single-molecule level.-
dc.language영어-
dc.publisherAMER CHEMICAL SOC-
dc.titleUncovering the Conformational Distribution of a Small Protein with Nanoparticle-Aided Cryo-Electron Microscopy Sampling-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000677581400015-
dc.identifier.scopusid2-s2.0-85111213249-
dc.identifier.rimsid76124-
dc.contributor.affiliatedAuthorHosung Ki-
dc.contributor.affiliatedAuthorJunbeom Jo-
dc.contributor.affiliatedAuthorYoungmin Kim-
dc.contributor.affiliatedAuthorTae Wu Kim-
dc.contributor.affiliatedAuthorChangin Kim-
dc.contributor.affiliatedAuthorSrinivasan Muniyappan-
dc.contributor.affiliatedAuthorSang Jin Lee-
dc.contributor.affiliatedAuthorYonggwan Kim-
dc.contributor.affiliatedAuthorHo Min Kim-
dc.contributor.affiliatedAuthorHyotcherl Ihee-
dc.identifier.doi10.1021/acs.jpclett.1c01277-
dc.identifier.bibliographicCitationJOURNAL OF PHYSICAL CHEMISTRY LETTERS, v.12, no.28, pp.6565 - 6573-
dc.relation.isPartOfJOURNAL OF PHYSICAL CHEMISTRY LETTERS-
dc.citation.titleJOURNAL OF PHYSICAL CHEMISTRY LETTERS-
dc.citation.volume12-
dc.citation.number28-
dc.citation.startPage6565-
dc.citation.endPage6573-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.relation.journalResearchAreaMaterials Science-
dc.relation.journalResearchAreaPhysics-
dc.relation.journalWebOfScienceCategoryChemistry, Physical-
dc.relation.journalWebOfScienceCategoryNanoscience & Nanotechnology-
dc.relation.journalWebOfScienceCategoryMaterials Science, Multidisciplinary-
dc.relation.journalWebOfScienceCategoryPhysics, Atomic, Molecular & Chemical-
dc.subject.keywordPlusINTRINSICALLY UNSTRUCTURED PROTEINS-
dc.subject.keywordPlusATOMIC-FORCE MICROSCOPY-
dc.subject.keywordPlusDISORDERED PROTEINS-
dc.subject.keywordPlusUNFOLDED STATE-
dc.subject.keywordPlusLIQUID-
Appears in Collections:
Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > 1. Journal Papers (저널논문)
Pioneer Research Center for Biomolecular and Cellular Structure(바이오분자 및 세포구조 연구단) > Protein Communication Group(단백질 커뮤니케이션 그룹) > 1. Journal Papers (저널논문)
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