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Kim, Jin Soo
유전체 교정 연구단
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Web-based CRISPR Toolkits: Cas-OFFinder, cas-designer, and cas-analyzer

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Title
Web-based CRISPR Toolkits: Cas-OFFinder, cas-designer, and cas-analyzer
Author(s)
Gue-Ho Hwang; Jin-Soo Kim; Sangsu Bae
Publication Date
2021-01
Journal
Methods in molecular biology (Clifton, N.J.), v.2162, pp.23 - 33
Publisher
Humana Press, Inc.
Abstract
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.The CRISPR-Cas system facilitates highly efficient genome editing; thus, it has been applied in many research fields such as biological science, medicine, and gene therapy. However, CRISPR nucleases can cleave off-target sites as well as on-target sites, causing unwanted mutations. Furthermore, after CRISPR treatments are delivered into cells or organisms, it is important to estimate the resulting mutation rates and to determine the patterns of mutations, but these tasks can be difficult. To address these issues, we have developed a tool for identifying potential off-target sites (Cas-OFFinder), a tool for designing CRISPR targets (Cas-Designer), and an assessment tool (Cas-Analyzer). These programs are all implemented on our website so that researchers can easily design CRISPR guide RNAs and assess the resulting mutations by simply clicking on the appropriate buttons; no login process is required
URI
https://pr.ibs.re.kr/handle/8788114/9821
DOI
10.1007/978-1-0716-0687-2_2
ISSN
1064-3745
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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