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Prelabeling Expansion Single-Molecule Localization Microscopy with Minimal Linkage Error

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Title
Prelabeling Expansion Single-Molecule Localization Microscopy with Minimal Linkage Error
Author(s)
Minsu Kang; Lee, Jooyong; Sangyoon Ko; Sang-HeeShim
Publication Date
2021-04
Journal
CHEMBIOCHEM, v.22, no.8, pp.1396 - 1399
Publisher
WILEY-V C H VERLAG GMBH
Abstract
Expansion microscopy combined with single-molecule localization microscopy (ExSMLM) has a potential for approaching molecular resolution. However, ExSMLM faces multiple challenges such as loss of fluorophores and proteins during polymerization, digestion or denaturation, and an increase in linkage error arising from the distance between the fluorophore and the target molecule. Here, we introduce a trifunctional streptavidin to link the target, fluorophore and gel matrix via a biotinylizable peptide tag. The resultant ExSMLM images of vimentin filaments demonstrated high labeling efficiency and a minimal linkage error of similar to 5 nm. Our ExSMLM provides a simple and practical means for fluorescence imaging with molecular resolution.
URI
https://pr.ibs.re.kr/handle/8788114/9557
DOI
10.1002/cbic.202000772
ISSN
1439-4227
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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