BROWSE

Related Scientist

RNA's photo.

RNA
RNA 연구단
more info

ITEM VIEW & DOWNLOAD

Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells

Cited 0 time in webofscience Cited 0 time in scopus
7 Viewed 0 Downloaded
Title
Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells
Author(s)
Kim, Chuna; Sung, Sanghyun; Jong-Seo Kim; Lee, Hyunji; Yoonseok Jung; Sanghee Shin; Kim, Eunkyeong; Jenny J. Seo; Kim, Jun; Kim, Daeun; Niida, Hiroyuki; V. Narry Kim; Park, Daechan; Lee, Junho
Publication Date
2021-02
Journal
NATURE COMMUNICATIONS, v.12, no.1
Publisher
NATURE RESEARCH
Abstract
Telomeres are part of a highly refined system for maintaining the stability of linear chromosomes. Most telomeres rely on simple repetitive sequences and telomerase enzymes to protect chromosomal ends; however, in some species or telomerase-defective situations, an alternative lengthening of telomeres (ALT) mechanism is used. ALT mainly utilises recombination-based replication mechanisms and the constituents of ALT-based telomeres vary depending on models. Here we show that mouse telomeres can exploit non-telomeric, unique sequences in addition to telomeric repeats. We establish that a specific subtelomeric element, the mouse template for ALT (mTALT), is used for repairing telomeric DNA damage as well as for composing portions of telomeres in ALT-dependent mouse embryonic stem cells. Epigenomic and proteomic analyses before and after ALT activation reveal a high level of non-coding mTALT transcripts despite the heterochromatic nature of mTALT-based telomeres. After ALT activation, the increased HMGN1, a non-histone chromosomal protein, contributes to the maintenance of telomere stability by regulating telomeric transcription. These findings provide a molecular basis to study the evolution of new structures in telomeres. Telomeres can be maintained by a telomerase-independent mechanism called an alternative lengthening of telomeres (ALT). Here the authors use mouse Terc (telomerase RNA) knockout embryonic cells and provide longitudinal analysis of ALT telomeres maintained with non-telomeric sequences.
URI
https://pr.ibs.re.kr/handle/8788114/9320
DOI
10.1038/s41467-021-21341-x
ISSN
2041-1723
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse