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나노의학 연구단
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Multiscale 3D phenotyping of human cerebral organoids

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Title
Multiscale 3D phenotyping of human cerebral organoids
Author(s)
Alexandre Albanese; Justin M. Swaney; Dae Hee Yun; Nicholas B. Evans; Jenna M. Antonucci; Silvia Velasco; Chang Ho Sohn; Paola Arlotta; Lee Gehrke; Kwanghun Chung
Subject
HUMAN BRAIN-DEVELOPMENT, ; ZIKA VIRUS, ; CELL DIVERSITY, ; HUMAN ES, ; DIFFERENTIATION, ; DYNAMICS, ; FEATURES
Publication Date
2020-12-08
Journal
SCIENTIFIC REPORTS, v.10, no.1, pp.1 - 17
Publisher
NATURE RESEARCH
Abstract
Brain organoids grown from human pluripotent stem cells self-organize into cytoarchitectures resembling the developing human brain. These three-dimensional models offer an unprecedented opportunity to study human brain development and dysfunction. Characterization currently sacrifices spatial information for single-cell or histological analysis leaving whole-tissue analysis mostly unexplored. Here, we present the SCOUT pipeline for automated multiscale comparative analysis of intact cerebral organoids. Our integrated technology platform can rapidly clear, label, and image intact organoids. Algorithmic- and convolutional neural network-based image analysis extract hundreds of features characterizing molecular, cellular, spatial, cytoarchitectural, and organoid-wide properties from fluorescence microscopy datasets. Comprehensive analysis of 46 intact organoids and similar to 100 million cells reveals quantitative multiscale "phenotypes" for organoid development, culture protocols and Zika virus infection. SCOUT provides a much-needed framework for comparative analysis of emerging 3D in vitro models using fluorescence microscopy.
URI
https://pr.ibs.re.kr/handle/8788114/9058
DOI
10.1038/s41598-020-78130-7
ISSN
2045-2322
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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