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Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device

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Title
Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device
Author(s)
Jiyong Cheong; Hojeong Yu; Chang Yeol Lee; Jung-uk Lee; Hyun-Jung Choi; Jae-Hyun Lee; Hakho Lee; Jinwoo Cheon
Publication Date
2020-12
Journal
Nature Biomedical Engineering, v.4, no.12, pp.1159 - 1167
Publisher
NATURE RESEARCH
Abstract
The diagnosis of severe acute respiratory syndrome 2 (SARS-CoV-2) infection by quantitative PCR with reverse transcription (RT–qPCR) typically involves bulky instrumentation in centralized laboratories and an assay time of 1–2 h. Here, we show that SARS-CoV-2 RNA can be detected in 17 min via a portable device integrating reverse transcription, fast thermocycling (via plasmonic heating through magneto-plasmonic nanoparticles) and in situ fluorescence detection following magnetic clearance of the nanoparticles. The device correctly classified all nasopharyngeal, oropharyngeal and sputum samples from 75 patients with COVID-19 and 75 healthy controls, with good concordance in fluorescence intensity with standard RT–qPCR (Pearson coefficients > 0.7 for the N1, N2 and RPP30 genes). Fast, portable and automated nucleic acid detection should facilitate testing at the point of care.
URI
https://pr.ibs.re.kr/handle/8788114/9042
DOI
10.1038/s41551-020-00654-0
ISSN
2157-846X
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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