BROWSE

Related Scientist

CMSD's photo.

CMSD
분자 분광학 및 동력학 연구단
more info

ITEM VIEW & DOWNLOAD

Laser scanning reflection-matrix microscopy for aberration-free imaging through intact mouse skull

Cited 0 time in webofscience Cited 0 time in scopus
6 Viewed 0 Downloaded
Title
Laser scanning reflection-matrix microscopy for aberration-free imaging through intact mouse skull
Author(s)
Yoon, Seokchan; Lee, Hojun; Hong, Jin Hee; Lim, Yong-Sik; Choi, Wonshik
Publication Date
2020-11
Journal
Nature Communications, v.11, no.1, pp.1 - 12
Publisher
NATURE RESEARCH
Abstract
A mouse skull is a barrier for high-resolution optical imaging because its thick and inhomogeneous internal structures induce complex aberrations varying drastically from position to position. Invasive procedures creating either thinned-skull or open-skull windows are often required for the microscopic imaging of brain tissues underneath. Here, we propose a label-free imaging modality termed laser scanning reflection-matrix microscopy for recording the amplitude and phase maps of reflected waves at non-confocal points as well as confocal points. The proposed method enables us to find and computationally correct up to 10,000 angular modes of aberrations varying at every 10 × 10 µm2 patch in the sample plane. We realized reflectance imaging of myelinated axons in vivo underneath an intact mouse skull, with an ideal diffraction-limited spatial resolution of 450 nm. Furthermore, we demonstrated through-skull two-photon fluorescence imaging of neuronal dendrites and their spines by physically correcting the aberrations identified from the reflection matrix
URI
https://pr.ibs.re.kr/handle/8788114/9007
DOI
10.1038/s41467-020-19550-x
ISSN
2041-1723
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse