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Kim, Chi-Ju
첨단연성물질 연구단
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Fully automated platelet isolation on a centrifugal microfluidic device for molecular diagnostics

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Title
Fully automated platelet isolation on a centrifugal microfluidic device for molecular diagnostics
Author(s)
Chi-Ju Kim; Dong Yeob Ki; Juhee Park; Vijaya Sunkara; Tae-Hyeong Kim; YooHong Min; Yoon-Kyoung Cho
Publication Date
2020-03
Journal
Lab on a Chip, v.20, no.5, pp.949 - 957
Publisher
Royal Society of Chemistry
Abstract
Platelets play crucial roles in hemostasis and immunity. Over the last decades, clinical evidence has revealed the significance of platelets as complementary biomarkers for the detection and treatment of various diseases, including cancer. Due to a lack of well standardized convenient isolation methods for platelets, pre-analytical factors such as complex handling procedures negatively impact the quality of the platelet samples, including overactivation, low purity, and poor reproducibility. This may lead to biased interpretation of various downstream analyses, such as proteomic and genomic analyses. Herein, we describe a fully automated lab-on-a-disc-based method of platelet isolation from a small volume of blood (<1 mL). This method provides higher yields (>4 folds) and purity (>99%) and lower platelet activation than the conventional method. Moreover, it was also superior in the detection of platelet-related RNAs CD41, PF4, and P2Y12 due to lower contamination with white blood cells
URI
https://pr.ibs.re.kr/handle/8788114/9005
DOI
10.1039/c9lc01140d
ISSN
1473-0197
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
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