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ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8

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Title
ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8
Author(s)
S. Chul Kwon; Harim Jang; Shen, Siyuan; S. Chan Baek; Kijun Kim; Jihye Yang; Jeesoo Kim; Jong-Seo Kim; Suman Wang; Shi, Yunyu; Li, Fudong; V. Narry Kim
Publication Date
2020-11
Journal
Nucleic acids research, v.48, no.19, pp.11097 - 11112
Publisher
Oxford University Press
Abstract
The microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate 'cluster assistance' in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway
URI
https://pr.ibs.re.kr/handle/8788114/8990
DOI
10.1093/nar/gkaa827
ISSN
0305-1048
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
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