ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8
DC Field | Value | Language |
---|---|---|
dc.contributor.author | S. Chul Kwon | - |
dc.contributor.author | Harim Jang | - |
dc.contributor.author | Shen, Siyuan | - |
dc.contributor.author | S. Chan Baek | - |
dc.contributor.author | Kijun Kim | - |
dc.contributor.author | Jihye Yang | - |
dc.contributor.author | Jeesoo Kim | - |
dc.contributor.author | Jong-Seo Kim | - |
dc.contributor.author | Suman Wang | - |
dc.contributor.author | Shi, Yunyu | - |
dc.contributor.author | Li, Fudong | - |
dc.contributor.author | V. Narry Kim | - |
dc.date.accessioned | 2021-01-06T01:50:10Z | - |
dc.date.accessioned | 2021-01-06T01:50:10Z | - |
dc.date.available | 2021-01-06T01:50:10Z | - |
dc.date.available | 2021-01-06T01:50:10Z | - |
dc.date.created | 2020-12-03 | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/8990 | - |
dc.description.abstract | The microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate 'cluster assistance' in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | Oxford University Press | - |
dc.title | ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000606018400040 | - |
dc.identifier.scopusid | 2-s2.0-85095799384 | - |
dc.identifier.rimsid | 73844 | - |
dc.contributor.affiliatedAuthor | S. Chul Kwon | - |
dc.contributor.affiliatedAuthor | Harim Jang | - |
dc.contributor.affiliatedAuthor | S. Chan Baek | - |
dc.contributor.affiliatedAuthor | Kijun Kim | - |
dc.contributor.affiliatedAuthor | Jihye Yang | - |
dc.contributor.affiliatedAuthor | Jeesoo Kim | - |
dc.contributor.affiliatedAuthor | Jong-Seo Kim | - |
dc.contributor.affiliatedAuthor | V. Narry Kim | - |
dc.identifier.doi | 10.1093/nar/gkaa827 | - |
dc.identifier.bibliographicCitation | Nucleic acids research, v.48, no.19, pp.11097 - 11112 | - |
dc.citation.title | Nucleic acids research | - |
dc.citation.volume | 48 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 11097 | - |
dc.citation.endPage | 11112 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Argonaute Proteins | - |
dc.subject.keywordAuthor | RNA-induced Silencing Complex | - |
dc.subject.keywordAuthor | Double-Stranded RNA Binding Motif | - |