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첨단연성물질연구단
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A lab-on-a-disc platform enables serial monitoring of individual CTCs associated with tumor progression during EGFR-targeted therapy for patients with NSCLC

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dc.contributor.authorMinji Lim-
dc.contributor.authorJuhee Park-
dc.contributor.authorAlarice C. Lowe-
dc.contributor.authorHyoung-oh Jeong-
dc.contributor.authorSemin Lee-
dc.contributor.authorHee Chul Park-
dc.contributor.authorKyusang Lee-
dc.contributor.authorGwang Ha Kim-
dc.contributor.authorMi-Hyun Kim-
dc.contributor.authorYoon-Kyoung Cho-
dc.date.accessioned2020-12-22T07:52:02Z-
dc.date.accessioned2020-12-22T07:52:02Z-
dc.date.available2020-12-22T07:52:02Z-
dc.date.available2020-12-22T07:52:02Z-
dc.date.created2020-06-29-
dc.date.issued2020-04-
dc.identifier.issn1838-7640-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/8744-
dc.description.abstract© The author(s). Rationale: Unlike traditional biopsy, liquid biopsy, which is a largely non-invasive diagnostic and monitoring tool, can be performed more frequently to better track tumors and mutations over time and to validate the efficiency of a cancer treatment. Circulating tumor cells (CTCs) are considered promising liquid biopsy biomarkers; however, their use in clinical settings is limited by high costs and a low throughput of standard platforms for CTC enumeration and analysis. In this study, we used a label-free, high-throughput method for CTC isolation directly from whole blood of patients using a standalone, clinical setting-friendly platform. Methods: A CTC-based liquid biopsy approach was used to examine the efficacy of therapy and emergent drug resistance via longitudinal monitoring of CTC counts, DNA mutations, and single-cell-level gene expression in a prospective cohort of 40 patients with epidermal growth factor receptor (EGFR)-mutant non-small cell lung cancer. Results: The change ratio of the CTC counts was associated with tumor response, detected by CT scan, while the baseline CTC counts did not show association with progression-free survival or overall survival. We achieved a 100% concordance rate for the detection of EGFR mutation, including emergence of T790M, between tumor tissue and CTCs. More importantly, our data revealed the importance of the analysis of the epithelial/mesenchymal signature of individual pretreatment CTCs to predict drug responsiveness in patients. Conclusion: The fluid-assisted separation technology disc platform enables serial monitoring of CTC counts, DNA mutations, as well as unbiased molecular characterization of individual CTCs associated with tumor progression during targeted therapy-
dc.description.uri1-
dc.language영어-
dc.publisherIVYSPRING INT PUBL-
dc.titleA lab-on-a-disc platform enables serial monitoring of individual CTCs associated with tumor progression during EGFR-targeted therapy for patients with NSCLC-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000526083000002-
dc.identifier.scopusid2-s2.0-85083823433-
dc.identifier.rimsid72196-
dc.contributor.affiliatedAuthorMinji Lim-
dc.contributor.affiliatedAuthorJuhee Park-
dc.contributor.affiliatedAuthorYoon-Kyoung Cho-
dc.identifier.doi10.7150/thno.44693-
dc.identifier.bibliographicCitationTHERANOSTICS, v.10, no.12, pp.5181 - 5194-
dc.citation.titleTHERANOSTICS-
dc.citation.volume10-
dc.citation.number12-
dc.citation.startPage5181-
dc.citation.endPage5194-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusLUNG-CANCER-
dc.subject.keywordPlusPROGNOSTIC-SIGNIFICANCE-
dc.subject.keywordPlusGENE-EXPRESSION-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusSURVIVAL-
dc.subject.keywordPlusBLOOD-
dc.subject.keywordPlusEMT-
dc.subject.keywordPlusOSIMERTINIB-
dc.subject.keywordPlusRESISTANCE-
dc.subject.keywordPlusINHIBITORS-
dc.subject.keywordAuthorcirculating tumor cells-
dc.subject.keywordAuthorsingle cell analysis-
dc.subject.keywordAuthorgene expression-
dc.subject.keywordAuthorEGFR mutation-
dc.subject.keywordAuthornon-small cell lung cancer-
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
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