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Shot-Noise-Limited Two-Color Stimulated Raman Scattering Microscopy with a Balanced Detection Scheme

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Title
Shot-Noise-Limited Two-Color Stimulated Raman Scattering Microscopy with a Balanced Detection Scheme
Author(s)
Youngjin Choi; Sohee Lim; Joong Won Shim; Bonghwan Chon; Jong Min Lim; Minhaeng Cho
Subject
Lipids,Microscopy,Power,Polarization,Lasers
Publication Date
2020-04
Journal
The journal of physical chemistry. B, v.124, no.13, pp.2591 - 2599
Publisher
ACS Publications
Abstract
© 2020 American Chemical Society Stimulated Raman scattering (SRS) microscopy has been considered a useful technique for investigating chemical components by selectively targeting the vibration mode of chemical structures. Its practical application to the observation of molecular structures and dynamics in complicated biological environments requires broad spectral coverage with both high resolution and a high signal-to-noise ratio. Here, we demonstrate a two-color SRS microscopy employing a balanced detection scheme and a spectral focusing method. Two different SRS signals are generated with pump and Stokes laser pulse pairs in perpendicular polarization, where each of them acts as an intensity reference for the other, significantly reducing the background noise level close to the shot-noise limit even with a fiber-based femtosecond laser system. The high spectral resolution comparable to that of spontaneous Raman scattering spectroscopy is achieved with the spectral focusing method. The two-color SRS images are obtained for a mixture of polymer beads and for the distributions of lipids and proteins in U2OS cells © 2020 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/8696
DOI
10.1021/acs.jpcb.0c01065
ISSN
1520-5207
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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