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Direct, sequence-specific detection of dsDNA based on peptide nucleic acid and graphene oxide without requiring denaturation

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Title
Direct, sequence-specific detection of dsDNA based on peptide nucleic acid and graphene oxide without requiring denaturation
Author(s)
Jieon Lee; Il-Soo Park; Jung, E; Lee, Y; Dal-Hee Min
Publication Date
2014-12
Journal
BIOSENSORS & BIOELECTRONICS, v.62, no., pp.140 - 144
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Abstract
Sequence-specific detectionofdoublestrandedDNA(dsDNA)isimportantinvariousresearch fields. In general,denaturationofdsDNAintosinglestrandsisnecessaryforthesequence-specific recognitionof probes totargetDNA,posingseveraldrawbackswhichdecreasetheefficiency asaDNAsensor.Herein, we reportadirect,sequence-specific dsDNAdetectionsystemwithoutrequiringanythermaldenaturing step. Ourstrategyutilizespeptidenucleicacid(PNA)andgrapheneoxide(GO)asaprobeandasa fluorescence quencher,respectively.ThePNA first bindstotheendofdsDNAstrandduetotherelatively easily dissociableterminalbasepairsofDNAduplex.Next,superiorbindingaffinity ofPNAtowards complementary DNAinducesbranchmigrationforgradualstrandreplacement,resultinginthe formation ofPNA/DNAduplex.UnlikeotherdsDNAsensorsbasedoncomplementaryDNAprobes,PNA in combinationwithGOenabledhybridizationwiththetargetsequencehiddenasaduplexformwithout denaturing stepandthus,theformationofPNA/DNAduplexwastranslatedintoselective fluorescence signal. Moreover,itprovidedtighterturn-onsignalcontrolwithverylowbackgroundsignalandhigh sensitivity andsequenceselectivityeveninthepresenceofserumproteins.
URI
https://pr.ibs.re.kr/handle/8788114/836
ISSN
0956-5663
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
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