IBS Publications Repository: Direct, sequence-specific detection of dsDNA based on peptide nucleic acid and graphene oxide without requiring denaturation

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IBS Publications RepositoryCenter for RNA Research(RNA 연구단)1. Journal Papers (저널논문)

Direct, sequence-specific detection of dsDNA based on peptide nucleic acid and graphene oxide without requiring denaturation

DC Field	Value	Language
dc.contributor.author	Jieon Lee	-
dc.contributor.author	Il-Soo Park	-
dc.contributor.author	Jung, E	-
dc.contributor.author	Lee, Y	-
dc.contributor.author	Dal-Hee Min	-
dc.date.available	2015-04-20T05:14:37Z	-
dc.date.created	2014-11-12	-
dc.date.issued	2014-12	-
dc.identifier.issn	0956-5663	-
dc.identifier.uri	https://pr.ibs.re.kr/handle/8788114/836	-
dc.description.abstract	Sequence-specific detectionofdoublestrandedDNA(dsDNA)isimportantinvariousresearch fields. In general,denaturationofdsDNAintosinglestrandsisnecessaryforthesequence-specific recognitionof probes totargetDNA,posingseveraldrawbackswhichdecreasetheefficiency asaDNAsensor.Herein, we reportadirect,sequence-specific dsDNAdetectionsystemwithoutrequiringanythermaldenaturing step. Ourstrategyutilizespeptidenucleicacid(PNA)andgrapheneoxide(GO)asaprobeandasa fluorescence quencher,respectively.ThePNA first bindstotheendofdsDNAstrandduetotherelatively easily dissociableterminalbasepairsofDNAduplex.Next,superiorbindingaffinity ofPNAtowards complementary DNAinducesbranchmigrationforgradualstrandreplacement,resultinginthe formation ofPNA/DNAduplex.UnlikeotherdsDNAsensorsbasedoncomplementaryDNAprobes,PNA in combinationwithGOenabledhybridizationwiththetargetsequencehiddenasaduplexformwithout denaturing stepandthus,theformationofPNA/DNAduplexwastranslatedintoselective fluorescence signal. Moreover,itprovidedtighterturn-onsignalcontrolwithverylowbackgroundsignalandhigh sensitivity andsequenceselectivityeveninthepresenceofserumproteins.	-
dc.description.uri	1	-
dc.language	영어	-
dc.publisher	ELSEVIER ADVANCED TECHNOLOGY	-
dc.subject	Biosensor	-
dc.subject	DNAdetection	-
dc.subject	DNAduplex	-
dc.subject	FRET	-
dc.subject	Graphene oxide	-
dc.subject	Peptide nucleicacid	-
dc.title	Direct, sequence-specific detection of dsDNA based on peptide nucleic acid and graphene oxide without requiring denaturation	-
dc.type	Article	-
dc.type.rims	ART	-
dc.identifier.wosid	000340334800023	-
dc.identifier.scopusid	2-s2.0-84903558578	-
dc.identifier.rimsid	16341	ko
dc.date.tcdate	2018-10-01	-
dc.contributor.affiliatedAuthor	Jieon Lee	-
dc.contributor.affiliatedAuthor	Il-Soo Park	-
dc.contributor.affiliatedAuthor	Dal-Hee Min	-
dc.identifier.doi	10.1016/j.bios.2014.06.028	-
dc.identifier.bibliographicCitation	BIOSENSORS & BIOELECTRONICS, v.62, pp.140 - 144	-
dc.citation.title	BIOSENSORS & BIOELECTRONICS	-
dc.citation.volume	62	-
dc.citation.startPage	140	-
dc.citation.endPage	144	-
dc.date.scptcdate	2018-10-01	-
dc.description.wostc	18	-
dc.description.scptc	19	-
dc.description.journalClass	1	-
dc.description.journalRegisteredClass	scie	-
dc.description.journalRegisteredClass	scopus	-
dc.subject.keywordPlus	DNA MISMATCH REPAIR	-
dc.subject.keywordPlus	POTENTIAL APPLICATIONS	-
dc.subject.keywordPlus	COLORIMETRIC DETECTION	-
dc.subject.keywordPlus	VIRUS	-
dc.subject.keywordPlus	CELLS	-
dc.subject.keywordPlus	RECOGNITION	-
dc.subject.keywordPlus	CARBON	-
dc.subject.keywordPlus	GENE	-
dc.subject.keywordPlus	PNA	-
dc.subject.keywordPlus	MUTATIONS	-
dc.subject.keywordAuthor	Biosensor	-
dc.subject.keywordAuthor	DNA detection	-
dc.subject.keywordAuthor	DNA duplex	-
dc.subject.keywordAuthor	FRET	-
dc.subject.keywordAuthor	Graphene oxide	-
dc.subject.keywordAuthor	Peptide nucleic acid	-