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복잡계자기조립연구단
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Bioreducible Polymer–delivered siRNA Targeting MMP-9: Suppression of Granulation Tissue Formation after Bare Metallic Stent Placement in a Rat Urethral Model

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dc.contributor.authorJung-Hoon Park-
dc.contributor.authorJin Hyoung Kim-
dc.contributor.authorEun-Young Kim-
dc.contributor.authorJinoo Kim-
dc.contributor.authorHo-Young Song-
dc.contributor.authorWon Jong Kim-
dc.contributor.authorDuhwan Lee-
dc.contributor.authorJihong Park-
dc.contributor.authorSoohwan Kim-
dc.date.available2015-04-19T10:57:44Z-
dc.date.created2014-11-24-
dc.date.issued2014-04-
dc.identifier.issn0033-8419-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/792-
dc.description.abstractTo evaluate the effectiveness of small interfering RNA (siRNA) targeting matrix metalloproteinase 9 (MMP-9) in suppressing granulation tissue formation caused by bare metallic stent placement in a rat urethral model. All experiments were approved by the committee of animal research. In 20 Sprague-Dawley male rats (weight range, 300–350 g), a self-expanding metallic bare stent was inserted in the urethra with fluoroscopic guidance. One group of 10 rats (group A) was treated with MMP-9 siRNA/bioreducible branched polyethylenimine–disulfide cross-linked–indocyanine green (bioreducible BPEI–SSICG), while the other group of 10 rats (group B) received control siRNA/bioreducible BPEI–SS-ICG treatment. All rats were sacrificed at 4 weeks. The therapeutic effectiveness of the MMP-9 siRNA/bioreducible BPEI–SS-ICG complex was assessed by comparing the two results of retrograde urethrography, histologic examination, and quantification of MMP-9 by using zymography and Western blot analysis between the two groups. The Mann-Whitney U test was used to evaluate differences. Stent placement was successful in all rats without a single case of migration at follow-up. Retrograde urethrography performed 4 weeks after stent placement demonstrated significantly larger luminal diameters of the urethra within the stents in group A compared with those in group B (P = .011). Histologic analysis revealed that the mean percentage of granulation tissue area (P , .001), mean number of epithelial layers (P , .001), and mean thickness of submucosal fibrosis (P , .001) were significantly decreased in group A compared with group B. Meanwhile, the mean density of inflammatory cell infiltration did not significantly differ between the two groups (P = .184). Quantitative analysis disclosed MMP-9 levels to be lower in group A relative to group B, indicating positive inhibition of MMP-9 by MMP-9 siRNA/bioreducible BPEI–SS-ICG. MMP-9 siRNA/bioreducible BPEI–SS-ICG is effective for inhibiting granulation tissue formation after bare metallic stent placement in a rat urethral model.-
dc.description.uri1-
dc.language영어-
dc.publisherRADIOLOGICAL SOC NORTH AMERICA-
dc.titleBioreducible Polymer–delivered siRNA Targeting MMP-9: Suppression of Granulation Tissue Formation after Bare Metallic Stent Placement in a Rat Urethral Model-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000335139200009-
dc.identifier.scopusid2-s2.0-84896972041-
dc.identifier.rimsid16534ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorWon Jong Kim-
dc.contributor.affiliatedAuthorDuhwan Lee-
dc.identifier.doi10.1148/radiol.13130980-
dc.identifier.bibliographicCitationRADIOLOGY, v.271, no.1, pp.87 - 95-
dc.citation.titleRADIOLOGY-
dc.citation.volume271-
dc.citation.number1-
dc.citation.startPage87-
dc.citation.endPage95-
dc.date.scptcdate2018-10-01-
dc.description.wostc3-
dc.description.scptc3-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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