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분자 분광학 및 동력학 연구단
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Three-dimensional interferometric scattering microscopy via remote focusing technique

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Title
Three-dimensional interferometric scattering microscopy via remote focusing technique
Author(s)
Il-Buem Lee; Hyeon-Min Moon; Jin-Sung Park; Katerina Zambochova; Seok-Cheol Hong; Minhaeng Cho
Subject
Three dimensional imaging,Image quality, Optical imaging
Publication Date
2020-05
Journal
Optics Letters, v.45, no.9, pp.2628 - 2631
Publisher
OSA Publishing
Abstract
© 2020 Optical Society of America.Interferometric scattering (iSCAT) microscopy enables us to track nm-sized objects with high spatial and temporal resolutions and permits label-free imaging of biomolecules. Its superb sensitivity, however, comes at a cost by several downsides, such as slow three-dimensional imaging and limited vertical tracking. Here, we propose a new method, Remote Focusing-iSCAT (RF-iSCAT) microscopy, to visualize a volume specimen by imaging sections at different depths without translation of either the objective lens or sample stage. We demonstrate the principle of RF-iSCAT by determining the z-position of submicrometer beads by translating the reference mirror instead. RF-iSCAT features an unprecedentedly long range of vertical tracking and permits fast but vibration-free vertical scanning. We anticipate that RF-iSCAT would enhance the utility of iSCAT for dynamics study
URI
https://pr.ibs.re.kr/handle/8788114/7873
DOI
10.1364/OL.386172
ISSN
0146-9592
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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