RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein

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Title
RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
Author(s)
Jeong-Soo Lee; Su-Jin Kwak; Jungeun Kim; Ae-Kyeong Kim; Hae Min Noh; Jin Soo Kim; Kweon Yu
Publication Date
2014-07
Journal
G3-GENES GENOMES GENETICS, v.4, no.7, pp.1291 - 1295
Publisher
GENETICS SOCIETY AMERICA
Abstract
We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
URI
https://pr.ibs.re.kr/handle/8788114/785
ISSN
2160-1836
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
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