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RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
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- Title
- RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
- Author(s)
- Jeong-Soo Lee; Su-Jin Kwak; Jungeun Kim; Ae-Kyeong Kim; Hae Min Noh; Jin Soo Kim; Kweon Yu
- Subject
- Cas9 protein, RGEN, Drosophila
- Publication Date
- 2014-07
- Journal
- G3-GENES GENOMES GENETICS, v.4, no.7, pp.1291 - 1295
- Publisher
- GENETICS SOCIETY AMERICA
- Abstract
- We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
- ISSN
- 2160-1836
- Appears in Collections:
- Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
- Files in This Item:
- RNA-Guided Genome Editing in Drosophila with.pdfDownload
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Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
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