RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong-Soo Lee | - |
dc.contributor.author | Su-Jin Kwak | - |
dc.contributor.author | Jungeun Kim | - |
dc.contributor.author | Ae-Kyeong Kim | - |
dc.contributor.author | Hae Min Noh | - |
dc.contributor.author | Jin Soo Kim | - |
dc.contributor.author | Kweon Yu | - |
dc.date.available | 2015-04-19T10:57:40Z | - |
dc.date.created | 2014-11-26 | - |
dc.date.issued | 2014-07 | - |
dc.identifier.issn | 2160-1836 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/785 | - |
dc.description.abstract | We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay. | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | GENETICS SOCIETY AMERICA | - |
dc.subject | Cas9 protein, RGEN, Drosophila | - |
dc.title | RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000339326600010 | - |
dc.identifier.scopusid | 2-s2.0-84904572233 | - |
dc.identifier.rimsid | 16560 | ko |
dc.date.tcdate | 2018-10-01 | - |
dc.contributor.affiliatedAuthor | Jin Soo Kim | - |
dc.identifier.doi | 10.1534/g3.114.012179 | - |
dc.identifier.bibliographicCitation | G3-GENES GENOMES GENETICS, v.4, no.7, pp.1291 - 1295 | - |
dc.citation.title | G3-GENES GENOMES GENETICS | - |
dc.citation.volume | 4 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1291 | - |
dc.citation.endPage | 1295 | - |
dc.date.scptcdate | 2018-10-01 | - |
dc.description.wostc | 22 | - |
dc.description.scptc | 25 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |