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Mechanical Adaptations of Epithelial Cells on Various Protruded Convex Geometries

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Title
Mechanical Adaptations of Epithelial Cells on Various Protruded Convex Geometries
Author(s)
Sun-Min Yu; Bo Li; Steve Granick; Yoon-Kyoung Cho
Subject
3D geometry, ; actin contractility, ; epithelial cell, ; keratin, ; mechanobiology, ; morphology, ; tubulin, ; vimentin
Publication Date
2020-06
Journal
Cells, v.9, no.6, pp.1434
Publisher
MDPI
Abstract
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The shape of epithelial tissue supports physiological functions of organs such as intestinal villi and corneal epithelium. Despite the mounting evidence showing the importance of geometry in tissue microenvironments, the current understanding on how it affects biophysical behaviors of cells is still elusive. Here, we cultured cells on various protruded convex structure such as triangle, square, and circle shape fabricated using two-photon laser lithography and quantitatively analyzed individual cells. Morphological data indicates that epithelial cells can sense the sharpness of the corner by showing the characteristic cell alignments, which was caused by actin contractility. Cell area was mainly influenced by surface convexity, and Rho-activation increased cell area on circle shape. Moreover, we found that intermediate filaments, vimentin, and cytokeratin 8/18, play important roles in growth and adaptation of epithelial cells by enhancing expression level on convex structure depending on the shape. In addition, microtubule building blocks, α-tubulin, was also responded on geometric structure, which indicates that intermediate filaments and microtubule can cooperatively secure mechanical stability of epithelial cells on convex surface. Altogether, the current study will expand our understanding of mechanical adaptations of cells on out-of-plane geometry
URI
https://pr.ibs.re.kr/handle/8788114/7834
DOI
10.3390/cells9061434
ISSN
2073-4409
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
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