CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice
DC Field | Value | Language |
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dc.contributor.author | Wang, CH | - |
dc.contributor.author | Lundh, M | - |
dc.contributor.author | Fu, A | - |
dc.contributor.author | Kriszt, R | - |
dc.contributor.author | Huang, TL | - |
dc.contributor.author | Lynes, MD | - |
dc.contributor.author | Leiria, LO | - |
dc.contributor.author | Shamsi, F | - |
dc.contributor.author | Darcy, J | - |
dc.contributor.author | Greenwood, BP | - |
dc.contributor.author | Narain, NR | - |
dc.contributor.author | Tolstikov, V | - |
dc.contributor.author | Smith, KL | - |
dc.contributor.author | Emanuelli, B | - |
dc.contributor.author | Young-Tae Chang | - |
dc.contributor.author | Hagen, S | - |
dc.contributor.author | Danial, NN | - |
dc.contributor.author | Kiebish, MA | - |
dc.contributor.author | Tseng, YH | - |
dc.date.accessioned | 2020-12-22T02:48:26Z | - |
dc.date.accessioned | 2020-12-22T02:48:26Z | - |
dc.date.available | 2020-12-22T02:48:26Z | - |
dc.date.available | 2020-12-22T02:48:26Z | - |
dc.date.created | 2020-10-16 | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 1946-6234 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/7694 | - |
dc.description.abstract | © 2020 The Authors, some rights reserved. Brown and brown-like beige/brite adipocytes dissipate energy and have been proposed as therapeutic targets to combat metabolic disorders. However, the therapeutic effects of cell-based therapy in humans remain unclear. Here, we created human brown-like (HUMBLE) cells by engineering human white preadipocytes using CRISPR-Cas9-SAM-gRNA to activate endogenous uncoupling protein 1 expression. Obese mice that received HUMBLE cell transplants showed a sustained improvement in glucose tolerance and insulin sensitivity, as well as increased energy expenditure. Mechanistically, increased arginine/nitric oxide (NO) metabolism in HUMBLE adipocytes promoted the production of NO that was carried by S-nitrosothiols and nitrite in red blood cells to activate endogenous brown fat and improved glucose homeostasis in recipient animals. Together, these data demonstrate the utility of using CRISPR-Cas9 technology to engineer human white adipocytes to display brown fat-like phenotypes and may open up cell-based therapeutic opportunities to combat obesity and diabetes | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | AMER ASSOC ADVANCEMENT SCIENCE | - |
dc.title | CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000563016800004 | - |
dc.identifier.scopusid | 2-s2.0-85089966226 | - |
dc.identifier.rimsid | 73062 | - |
dc.contributor.affiliatedAuthor | Young-Tae Chang | - |
dc.identifier.doi | 10.1126/scitranslmed.aaz8664 | - |
dc.identifier.bibliographicCitation | SCIENCE TRANSLATIONAL MEDICINE, v.12, no.558, pp.eaaz8664 | - |
dc.citation.title | SCIENCE TRANSLATIONAL MEDICINE | - |
dc.citation.volume | 12 | - |
dc.citation.number | 558 | - |
dc.citation.startPage | eaaz8664 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | WHITE ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | SKELETAL-MUSCLE | - |
dc.subject.keywordPlus | ENERGY-EXPENDITURE | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | FAT | - |
dc.subject.keywordPlus | COLD | - |
dc.subject.keywordPlus | HEMOGLOBIN | - |
dc.subject.keywordPlus | HOMEOSTASIS | - |