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Chang, Young Tae
복잡계 자기조립 연구단
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CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice

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dc.contributor.authorWang, CH-
dc.contributor.authorLundh, M-
dc.contributor.authorFu, A-
dc.contributor.authorKriszt, R-
dc.contributor.authorHuang, TL-
dc.contributor.authorLynes, MD-
dc.contributor.authorLeiria, LO-
dc.contributor.authorShamsi, F-
dc.contributor.authorDarcy, J-
dc.contributor.authorGreenwood, BP-
dc.contributor.authorNarain, NR-
dc.contributor.authorTolstikov, V-
dc.contributor.authorSmith, KL-
dc.contributor.authorEmanuelli, B-
dc.contributor.authorYoung-Tae Chang-
dc.contributor.authorHagen, S-
dc.contributor.authorDanial, NN-
dc.contributor.authorKiebish, MA-
dc.contributor.authorTseng, YH-
dc.date.accessioned2020-12-22T02:48:26Z-
dc.date.accessioned2020-12-22T02:48:26Z-
dc.date.available2020-12-22T02:48:26Z-
dc.date.available2020-12-22T02:48:26Z-
dc.date.created2020-10-16-
dc.date.issued2020-08-
dc.identifier.issn1946-6234-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/7694-
dc.description.abstract© 2020 The Authors, some rights reserved. Brown and brown-like beige/brite adipocytes dissipate energy and have been proposed as therapeutic targets to combat metabolic disorders. However, the therapeutic effects of cell-based therapy in humans remain unclear. Here, we created human brown-like (HUMBLE) cells by engineering human white preadipocytes using CRISPR-Cas9-SAM-gRNA to activate endogenous uncoupling protein 1 expression. Obese mice that received HUMBLE cell transplants showed a sustained improvement in glucose tolerance and insulin sensitivity, as well as increased energy expenditure. Mechanistically, increased arginine/nitric oxide (NO) metabolism in HUMBLE adipocytes promoted the production of NO that was carried by S-nitrosothiols and nitrite in red blood cells to activate endogenous brown fat and improved glucose homeostasis in recipient animals. Together, these data demonstrate the utility of using CRISPR-Cas9 technology to engineer human white adipocytes to display brown fat-like phenotypes and may open up cell-based therapeutic opportunities to combat obesity and diabetes-
dc.description.uri1-
dc.language영어-
dc.publisherAMER ASSOC ADVANCEMENT SCIENCE-
dc.titleCRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000563016800004-
dc.identifier.scopusid2-s2.0-85089966226-
dc.identifier.rimsid73062-
dc.contributor.affiliatedAuthorYoung-Tae Chang-
dc.identifier.doi10.1126/scitranslmed.aaz8664-
dc.identifier.bibliographicCitationSCIENCE TRANSLATIONAL MEDICINE, v.12, no.558, pp.eaaz8664-
dc.citation.titleSCIENCE TRANSLATIONAL MEDICINE-
dc.citation.volume12-
dc.citation.number558-
dc.citation.startPageeaaz8664-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.subject.keywordPlusNITRIC-OXIDE SYNTHASE-
dc.subject.keywordPlusWHITE ADIPOSE-TISSUE-
dc.subject.keywordPlusSKELETAL-MUSCLE-
dc.subject.keywordPlusENERGY-EXPENDITURE-
dc.subject.keywordPlusINSULIN-RESISTANCE-
dc.subject.keywordPlusGENE-EXPRESSION-
dc.subject.keywordPlusFAT-
dc.subject.keywordPlusCOLD-
dc.subject.keywordPlusHEMOGLOBIN-
dc.subject.keywordPlusHOMEOSTASIS-
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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