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An improved surface passivation method for single-molecule studies
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- Title
- An improved surface passivation method for single-molecule studies
- Author(s)
- Hua, BY; Han, KY; Zhou, RB; Hajin Kim; Shi, XH; Abeysirigunawardena, SC; Jain, A; Singh, D; Aggarwal, V; Woodson, SA; Ha, T
- Publication Date
- 2014-12
- Journal
- NATURE METHODS, v.11, no.12, pp.1233 - 1239
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- We report a surface passivation method based on dichlorodimethylsilane (DDSDDSDDS)–Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDSDDSDDS–Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
- ISSN
- 1548-7091
- Appears in Collections:
- Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
- Files in This Item:
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