BROWSE

Related Scientist

Researcher

분자 분광학 및 동력학 연구단
분자 분광학 및 동력학 연구단
more info

Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy

Cited 0 time in webofscience Cited 0 time in scopus
6 Viewed 3 Downloaded
Title
Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy
Author(s)
Jiwoong Kwon; Park, Jong-Seok; Minsu Kang; Choi, Soobin; Park, Jumi; Kim, Gyeong Tae; Lee, Changwook; Cha, Sangwon; Rhee, Hyun-Woo; Sang-Hee Shim
Publication Date
2020-01
Journal
NATURE COMMUNICATIONS, v.11, no.1, pp.273 -
Publisher
NATURE PUBLISHING GROUP
Abstract
We introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-resolution imaging with photon numbers equivalent to the brightest photoswitchable red protein. UnaG only fluoresces upon binding of a fluorogenic metabolite, bilirubin, enabling UV-free reversible photoswitching with easily controllable kinetics and low background under Epi illumination. The on- and off-switching rates are controlled by the concentration of the ligand and the excitation light intensity, respectively, where the dissolved oxygen also promotes the off-switching. The photo-oxidation reaction mechanism of bilirubin in UnaG suggests that the lack of ligand-protein covalent bond allows the oxidized ligand to detach from the protein, emptying the binding cavity for rebinding to a fresh ligand molecule. We demonstrate super-resolution single-molecule localization imaging of various subcellular structures genetically encoded with UnaG, which enables facile labeling and simultaneous multicolor imaging of live cells. UnaG has the promise of becoming a default protein for high-performance super-resolution imaging. © The Author(s) 2020
URI
https://pr.ibs.re.kr/handle/8788114/7254
ISSN
2041-1723
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > Journal Papers (저널논문)
Files in This Item:
Bright ligand-activatable fluorescent protein_권지웅(심상희).pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse