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나노물질 및 화학반응 연구단
나노물질 및 화학반응 연구단
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Effect of Occluded Ligand Migration on the Kinetics and Structural Dynamics of Homodimeric Hemoglobin

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Title
Effect of Occluded Ligand Migration on the Kinetics and Structural Dynamics of Homodimeric Hemoglobin
Author(s)
Hanui Kim; Jong Goo Kim; Srinivasan Muniyappan; Tae Wu Kim; Sang Jin Lee; Hyotcherl Ihee
Publication Date
2020-02
Journal
JOURNAL OF PHYSICAL CHEMISTRY B, v.124, no.8, pp.1550 - 1556
Publisher
AMER CHEMICAL SOC
Abstract
© 2020 American Chemical Society.Small molecules such as molecular oxygen, nitric oxide, and carbon monoxide play important roles in life, and many proteins require the transport of small molecules to and from the bulk solvent for their function. Ligand migration within a protein molecule is expected to be closely related to the overall structural changes of the protein, but the detailed and quantitative connection remains elusive. For example, despite numerous studies, how occluded ligand migration affects the kinetics and structural dynamics of the R-T transition remains unclear. To shed light on this issue, we chose homodimeric hemoglobin (HbI) with the I114F mutation (I114F), which is known to interfere with ligand migration between the primary and secondary docking sites, and studied its kinetics and structural dynamics using time-resolved X-ray solution scattering. The kinetic analysis shows that I114F has three structurally distinct intermediates (I1, I2, and I3) as in the wild type (WT), but its geminate CO recombination occurs directly from I1 without the path via I2 observed in WT. Moreover, the structural transitions, which involve ligand migration (the transitions from I1 to I2 and from I3 to the initial state), are decelerated compared to WT. The structural analysis revealed that I114F involves generally smaller structural changes in all three intermediates compared to WT
URI
https://pr.ibs.re.kr/handle/8788114/7232
ISSN
1520-6106
Appears in Collections:
Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > Journal Papers (저널논문)
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