An inducible system for in vitro and in vivo Fas activation using FKBP-FRB-rapamycin complex

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Title
An inducible system for in vitro and in vivo Fas activation using FKBP-FRB-rapamycin complex
Author(s)
Seokhwi Kim; Jongpil Shin; Hyunsik Oh; Sangphil Ahn; Nury Kim; Won Do Heo
Publication Date
2020-03
Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.523, no.2, pp.473 - 480
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract
© 2019 Elsevier Inc.The inducible activation system is valuable for investigating spatiotemporal roles of molecules. A chemically inducible activation system for Fas (CD95/APO-1), which works efficiently to induce apoptosis and leads non-apoptotic pathways, has not yet been developed. Here, we engineered a rapamycin-induced dimerization system of Fas consisting of FKBP and FRB proteins. Treatment of rapamycin specifically induces cellular apoptosis. In neurons and cells with high c-FLIP expression, rapamycin-induced Fas activation triggered the activation of the non-apoptotic pathway components instead of cell death. Intracranial delivery of the system could be utilized to induce apoptosis of tumor cells upon rapamycin treatment. Our results demonstrate a novel inducible Fas activation system which operates with high efficiency and temporal precision in vitro and in vivo promising a potential therapeutic strategy
URI
https://pr.ibs.re.kr/handle/8788114/7223
ISSN
0006-291X
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Social Neuroscience Group(사회성 뇌과학 그룹) > Journal Papers (저널논문)
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
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