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Optogenetic control of mRNA localization and translation in live cells

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Title
Optogenetic control of mRNA localization and translation in live cells
Author(s)
Na Yeon Kim; Sangkyu Lee; Jeonghye Yu; Nury Kim; Seong Su Won; Hyerim Park; Won Do Heo
Publication Date
2020-02
Journal
Nature Cell Biology, v.22, no.3, pp.341 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Despite efforts to visualize the spatio–temporal dynamics of single messenger RNAs, the ability to precisely control their function has lagged. This study presents an optogenetic approach for manipulating the localization and translation of specific mRNAs by trapping them in clusters. This clustering greatly amplified reporter signals, enabling endogenous RNA–protein interactions to be clearly visualized in single cells. Functionally, this sequestration reduced the ability of mRNAs to access ribosomes, markedly attenuating protein synthesis. A spatio–temporally resolved analysis indicated that sequestration of endogenous β-actin mRNA attenuated cell motility through the regulation of focal-adhesion dynamics. These results suggest a mechanism highlighting the indispensable role of newly synthesized β-actin protein for efficient cell migration. This platform may be broadly applicable for use in investigating the spatio–temporal activities of specific mRNAs in various biological processes.
URI
https://pr.ibs.re.kr/handle/8788114/7185
ISSN
1465-7392
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Social Neuroscience Group(사회성 뇌과학 그룹) > Journal Papers (저널논문)
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
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