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유전체 항상성 연구단
유전체 항상성 연구단
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Simple Method to Characterize the Ciliary Proteome of Multiciliated Cells

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Title
Simple Method to Characterize the Ciliary Proteome of Multiciliated Cells
Author(s)
Sim H.J.; Yun S.; Kim H.E.; Kwon K.Y.; Kim G.-H.; Yun S.; Byung Gyu Kim; Kyungjae Myung; Tae Joo Park; Taejoon Kwon
Publication Date
2020-01
Journal
JOURNAL OF PROTEOME RESEARCH, v.19, no.1, pp.391 - 400
Publisher
AMER CHEMICAL SOC
Abstract
Copyright © 2019 American Chemical Society.Motile cilia of multiciliated epithelial cells have important roles in animal development and cell homeostasis. Although several studies have identified and reported proteins localized in this complex organelle and the related immotile primary cilia from various cell types, it is still challenging to isolate high quantities of ciliary proteins for proteomic analysis. In this study, African clawed frog (Xenopus laevis) embryos, which have many multiciliated cells in the epidermis, were treated with a simple ionic buffer to identify 1009 proteins conserved across vertebrates; these proteins were putatively localized in motile cilia. Using two ciliary proteome databases, we confirmed that previously validated cilia-associated proteins are highly enriched in our ciliary proteome. Proteins localized at the transition zone and Ellis-van Creveld zone, which are distinct regions at the base of cilia, near the junction with the apical cell surface, were isolated using our method. Among the newly identified ciliary proteins, we report that KRT17 may have an unrecognized function in motile cilia. Hence, the method developed in this study would be useful for understanding the ciliary proteome
URI
https://pr.ibs.re.kr/handle/8788114/7043
ISSN
1535-3893
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > Journal Papers (저널논문)
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