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Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRAC(swell)) in the Brain

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dc.contributor.authorYoung-Eun Han-
dc.contributor.authorJea Kwon-
dc.contributor.authorJoungha Won-
dc.contributor.authorHeeyoung An-
dc.contributor.authorMinwoo Wendy Jang-
dc.contributor.authorWoo, J-
dc.contributor.authorLee, JS-
dc.contributor.authorMin Gu Park-
dc.contributor.authorYoon, BE-
dc.contributor.authorLee, SE-
dc.contributor.authorHwang, EM-
dc.contributor.authorJung, JY-
dc.contributor.authorPark, H-
dc.contributor.authorOh, SJ-
dc.contributor.authorC. Justin Lee-
dc.date.available2020-01-31T00:56:28Z-
dc.date.created2019-06-17-
dc.date.issued2019-04-
dc.identifier.issn1226-2560-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/6933-
dc.description.abstractIn the brain, a reduction in extracellular osmolality causes water-influx and swelling, which subsequently triggers Cl - and osmolytes-efflux via volume-regulated anion channel (VRAC). Although LRRC8 family has been recently proposed as the pore-forming VRAC which is activated by low cytoplasmic ionic strength but not by swelling, the molecular identity of the pore-forming swelling-dependent VRAC (VRAC(swell)) remains unclear. Here we identify and characterize. Tweety- homologs (TTYH1, TTYH2, TTYH3) as the major VRAC(swell) in astrocytes. Gene-silencing of all Ttyh1/2/3 eliminated hypo-osmotic-solution-induced Cl- conductance (I-Cl,I-swell) in cultured and hippocainpal astrocytes. When heterologously expressed in HEK293T or CHO-K1 cells, each TTYH iso-form showed a significant I-Cl,I-swell with similar aquaporin-4 dependency, pharmacological properties and glutamate permeability as I-Cl,I-swell observed in native astrocres. Mutagenesis-based structure-activity analysis revealed that positively charged arginine residue at 165 in TTYH1 and 164 in TTYH2 is critical for the formation of the channel-pore. Our results demonstrate that TTYH family confers the bona fide VRAC(swell) in the brain. © Experimental Neurobiology 2019.-
dc.description.uri1-
dc.language영어-
dc.publisherKOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE-
dc.titleTweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRAC(swell)) in the Brain-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000468151200006-
dc.identifier.scopusid2-s2.0-85066908958-
dc.identifier.rimsid68494-
dc.contributor.affiliatedAuthorYoung-Eun Han-
dc.contributor.affiliatedAuthorJea Kwon-
dc.contributor.affiliatedAuthorJoungha Won-
dc.contributor.affiliatedAuthorHeeyoung An-
dc.contributor.affiliatedAuthorMinwoo Wendy Jang-
dc.contributor.affiliatedAuthorMin Gu Park-
dc.contributor.affiliatedAuthorC. Justin Lee-
dc.identifier.doi10.5607/en.2019.28.2.183-
dc.identifier.bibliographicCitationEXPERIMENTAL NEUROBIOLOGY, v.28, no.2, pp.183 - 215-
dc.citation.titleEXPERIMENTAL NEUROBIOLOGY-
dc.citation.volume28-
dc.citation.number2-
dc.citation.startPage183-
dc.citation.endPage215-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.subject.keywordPlusSENSITIVE CL-CHANNEL-
dc.subject.keywordPlusCHLORIDE CURRENTS-
dc.subject.keywordPlusGLUTAMATE RELEASE-
dc.subject.keywordPlusASTROCYTES-
dc.subject.keywordPlusROLES-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusCONDUCTANCE-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusNEURONS-
dc.subject.keywordPlusLRRC8A-
dc.subject.keywordAuthorVolume -regulated anion channel-
dc.subject.keywordAuthorVRAC-
dc.subject.keywordAuthorTweety-homolog-
dc.subject.keywordAuthorTtyh-
dc.subject.keywordAuthorVolume regulation-
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > 1. Journal Papers (저널논문)
Center for Cognition and Sociality(인지 및 사회성 연구단) > Cognitive Glioscience Group(인지 교세포과학 그룹) > 1. Journal Papers (저널논문)
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Tweety-homolog (Ttyh) family encodes the pore-forming subunits of the swelling-dependent volume-regulated anion channel (VRACswell) in the brain.pdfDownload

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