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나노의학 연구단
나노의학 연구단
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Single-cell RNA-sequencing of migratory breast cancer cells: Discovering genes associated with cancer metastasis

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Title
Single-cell RNA-sequencing of migratory breast cancer cells: Discovering genes associated with cancer metastasis
Author(s)
Yu-Chih Chen; Saswat Sahoo; Riley Brien; Seungwon Jung; Brock Humphries; Woncheol Lee; Yu-Heng Cheng; Zhixiong Zhang; Kathryn E. Luker; Max S. Wicha; Gary D. Luker; Euisik Yoon
Publication Date
2019-12
Journal
ANALYST, v.144, no.24, pp.7296 - 7309
Publisher
ROYAL SOC CHEMISTRY
Abstract
© 2019 The Royal Society of Chemistry.Considerable evidence suggests breast cancer metastasis arises from cells undergoing epithelial-to-mesenchymal-transition (EMT) and cancer stem-like cells (CSCs). Using a microfluidic device that enriches migratory breast cancer cells with enhanced capacity for tumor formation and metastasis, we identified genes differentially expressed in migratory cells by high-throughput single-cell RNA-sequencing. Migratory cells exhibited overall signatures of EMT and CSCs with variable expression of marker genes, and they retained expression profiles of EMT over time. With single-cell resolution, we discovered intermediate EMT states and distinct epithelial and mesenchymal sub-populations of migratory cells, indicating breast cancer cells can migrate rapidly while retaining an epithelial state. Migratory cells showed differential profiles for regulators of oxidative stress, mitochondrial morphology, and the proteasome, revealing potential vulnerabilities and unexpected consequences of drugs. We also identified novel genes correlated with cell migration and outcomes in breast cancer as potential prognostic biomarkers and therapeutic targets to block migratory cells in metastasis
URI
https://pr.ibs.re.kr/handle/8788114/6733
ISSN
0003-2654
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > Journal Papers
Files in This Item:
201912_Single-cell RNA-sequencing of migratory breast cancer cells Discovering genes associated with cancer metastasis.pdfDownload

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