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유전체 항상성 연구단
유전체 항상성 연구단
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Molecular characteristics of reiterative DNA unwinding by the Caenorhabditis elegans RecQ helicase

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Title
Molecular characteristics of reiterative DNA unwinding by the Caenorhabditis elegans RecQ helicase
Author(s)
Choi S.; Lee S.-W.; Hajin Kim; Ahn B.
Publication Date
2019-10
Journal
NUCLEIC ACIDS RESEARCH, v.47, no.18, pp.9708 - 9720
Publisher
OXFORD UNIV PRESS
Abstract
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.The RecQ family of helicases is highly conserved both structurally and functionally from bacteria to humans. Defects in human RecQ helicases are associated with genetic diseases that are characterized by cancer predisposition and/or premature aging. RecQ proteins exhibit 3'-5' helicase activity and play critical roles in genome maintenance. Recent advances in single-molecule techniques have revealed the reiterative unwinding behavior of RecQ helicases. However, the molecular mechanisms involved in this process remain unclear, with contradicting reports. Here, we characterized the unwinding dynamics of the Caenorhabditis elegans RecQ helicase HIM-6 using single-molecule fluorescence resonance energy transfer measurements. We found that HIM-6 exhibits reiterative DNA unwinding and the length of DNA unwound by the helicase is sharply defined at 25-31 bp. Experiments using various DNA substrates revealed that HIM-6 utilizes the mode of 'sliding back' on the translocated strand, without strand-switching for rewinding. Furthermore, we found that Caenorhabditis elegans replication protein A, a single-stranded DNA binding protein, suppresses the reiterative behavior of HIM-6 and induces unidirectional, processive unwinding, possibly through a direct interaction between the proteins. Our findings shed new light on the mechanism of DNA unwinding by RecQ family helicases and their co-operation with RPA in processing DNA
URI
https://pr.ibs.re.kr/handle/8788114/6412
ISSN
0305-1048
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > Journal Papers (저널논문)
Files in This Item:
NAR_2019_Molecular characteristics of reiterative DNA unwinding by the Caenorhabditis elegans RecQ helicase.pdfDownload

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