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김진수
유전체 교정 연구단
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Improving CRISPR Genome Editing by Engineering Guide RNAs

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Title
Improving CRISPR Genome Editing by Engineering Guide RNAs
Author(s)
Su Bin Moon; Do Yon Kim; Jeong-Heon Ko; Jin-soo Kim; Yong-Sam Kim
Publication Date
2019-08
Journal
TRENDS IN BIOTECHNOLOGY, v.37, no.8, pp.870 - 881
Publisher
ELSEVIER SCIENCE LONDON
Abstract
CRISPR technology is a two-component gene editing system in which the effector protein induces genetic alterations with the aid of a gene targeting guide RNA. Guide RNA can be produced through chemical synthesis, in vitro transcription, or intracellular transcription. Guide RNAs can be engineered to have chemical modifications, alterations in the spacer length, sequence modifications, fusion of RNA or DNA components, and incorporation of deoxynucleotides. Engineered guide RNA can improve genome editing efficiency and target specificity, regulation of biological toxicity, sensitive and specific molecular imaging, multiplexing, and editing flexibility. Therefore, engineered guide RNA will enable more specific, efficient, and safe gene editing, ultimately improving the clinical benefits of gene therapy. © 2019 Elsevier Ltd.
URI
https://pr.ibs.re.kr/handle/8788114/6367
ISSN
0167-7799
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
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