Improving CRISPR Genome Editing by Engineering Guide RNAs
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Su Bin Moon | - |
dc.contributor.author | Do Yon Kim | - |
dc.contributor.author | Jeong-Heon Ko | - |
dc.contributor.author | Jin-soo Kim | - |
dc.contributor.author | Yong-Sam Kim | - |
dc.date.available | 2019-10-22T07:38:05Z | - |
dc.date.created | 2019-03-19 | - |
dc.date.issued | 2019-08 | - |
dc.identifier.issn | 0167-7799 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/6367 | - |
dc.description.abstract | CRISPR technology is a two-component gene editing system in which the effector protein induces genetic alterations with the aid of a gene targeting guide RNA. Guide RNA can be produced through chemical synthesis, in vitro transcription, or intracellular transcription. Guide RNAs can be engineered to have chemical modifications, alterations in the spacer length, sequence modifications, fusion of RNA or DNA components, and incorporation of deoxynucleotides. Engineered guide RNA can improve genome editing efficiency and target specificity, regulation of biological toxicity, sensitive and specific molecular imaging, multiplexing, and editing flexibility. Therefore, engineered guide RNA will enable more specific, efficient, and safe gene editing, ultimately improving the clinical benefits of gene therapy. © 2019 Elsevier Ltd. | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCIENCE LONDON | - |
dc.subject | CRISPR | - |
dc.subject | gene therapy | - |
dc.subject | genome editing | - |
dc.subject | guide RNA | - |
dc.subject | guide RNA engineering | - |
dc.title | Improving CRISPR Genome Editing by Engineering Guide RNAs | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000474720000010 | - |
dc.identifier.scopusid | 2-s2.0-85062291194 | - |
dc.identifier.rimsid | 67624 | - |
dc.contributor.affiliatedAuthor | Jin-soo Kim | - |
dc.identifier.doi | 10.1016/j.tibtech.2019.01.009 | - |
dc.identifier.bibliographicCitation | TRENDS IN BIOTECHNOLOGY, v.37, no.8, pp.870 - 881 | - |
dc.citation.title | TRENDS IN BIOTECHNOLOGY | - |
dc.citation.volume | 37 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 870 | - |
dc.citation.endPage | 881 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | CHEMICAL-MODIFICATIONS | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | GENE KNOCKOUT | - |
dc.subject.keywordPlus | CAS9 | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | CPF1 | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | BASE | - |
dc.subject.keywordPlus | ENDONUCLEASES | - |
dc.subject.keywordAuthor | CRISPR | - |
dc.subject.keywordAuthor | gene therapy | - |
dc.subject.keywordAuthor | genome editing | - |
dc.subject.keywordAuthor | guide RNA | - |
dc.subject.keywordAuthor | guide RNA engineering | - |